Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4373 |
| PDBID: | 7MYZ |
| Chains: | IM_D |
| Organism: | Homo sapiens, Escherichia coli |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1038/s41467-021-25475-w |
| Antibody | |
| Antibody: | B6H12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Fusion protein of Leukocyte surface antigen CD47 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >7MYZ_I|Chain C[auth H], D[auth I]|B6H12 Fab heavy chain|Homo sapiens (9606) EVKLVESGGDLVKPGGSLKLSCAASGFTFSGYGMSWVRQTPDKRLEWVATITSGGTYTYYPDSVKGRFTISRDNAKNTLYLQIDSLKSEDTAIYFCARSLAGNAMDYWGQGTSVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS |
Light Chain: M
Mutation: NULL
| >7MYZ_M|Chain E[auth L], F[auth M]|B6H12 Fab light chain|Homo sapiens (9606) DIVMTQSPATLSVTPGDRVSLSCRASQTISDYLHWYQQKSHESPRLLIKFASQSISGIPSRFSGSGSGSDFTLSINSVEPEDVGVYYCQNGHGFPRTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: D
Mutation: NULL
| >7MYZ_D|Chain A[auth C], B[auth D]|Fusion protein of Leukocyte surface antigen CD47 an Soluble cytochrome b562|Homo sapiens (9606) QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDEHHHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition I: GLY31 TYR32 GLY33 TRP47 THR50 THR52 SER53 GLY54 THR56 TYR57 TYR59 SER99 LEU100 ALA101 GLY102 ASN103 M: SER30 ASP31 TYR32 LYS49 PHE50 GLN53 HIS92 GLY93 PHE94 ARG96 D: MET28 GLU29 GLN31 ASN32 THR34 GLU35 VAL36 TYR37 LYS39 LYS41 ASP46 ASP51 ALA53 LEU54 LYS56 GLU97 THR99 GLU100 LEU101 THR102 ARG103 GLU104 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
