Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4362 |
| PDBID: | 7MXL |
| Chains: | JK_C |
| Organism: | Homo sapiens, Betula pendula |
| Method: | EM |
| Resolution (Å): | 3.20 |
| Reference: | 10.1016/j.jaci.2021.05.038 |
| Antibody | |
| Antibody: | REGN5715 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Atisnolerbart(Phase-III) |
| Antigen | |
| Antigen: | Major pollen allergen Bet v 1-A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >7MXL_J|Chain D[auth J]|REGN5715 antibody Fab fragment heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYTFISYNIFWVRQATGQGLDWMGWMNPFRNNAGYAQKFQGRVTVTWDTSISTAYMELSSLSSEDTAIYYCAREHGSSWGFFDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPP |
Light Chain: K
Mutation: NULL
| >7MXL_K|Chain E[auth K]|REGN5715 antibody Fab fragment light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >7MXL_C|Chain C|Major pollen allergen Bet v 1-A|Betula pendula (3505) MGVFNYETETTSVIPAARLFKAFILDGDNLFPKVAPQAISSVENIEGNGGPGTIKKISFPEGFPFKYVKDRVDEVDHTNFKYNYSVIEGGPIGDTLEKISNEIKIVATPDGGSILKISNKYHTKGDHEVKAEQVKASKEMGETLLRAVESYLLAHSDAYN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition J: THR28 ILE30 SER31 TYR32 ASN33 ASN52 PHE54 ARG55 ARG98 HIS100 GLY101 SER102 SER103 TRP104 K: TYR33 C: LEU25 ASP26 ASP28 ASN29 GLU43 ASN44 ILE45 GLU46 GLY47 ASN48 GLY49 GLY50 PRO51 GLY52 THR53 LYS56 LYS69 VAL75 HIS77 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
