Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4361 |
| PDBID: | 7MXL |
| Chains: | HL_C |
| Organism: | Homo sapiens, Betula pendula |
| Method: | EM |
| Resolution (Å): | 3.20 |
| Reference: | 10.1016/j.jaci.2021.05.038 |
| Antibody | |
| Antibody: | REGN5714 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Umesolerbart(Phase-III) |
| Antigen | |
| Antigen: | Major pollen allergen Bet v 1-A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7MXL_H|Chain G[auth H]|REGN5714 antibody Fab fragment heavy chain|Homo sapiens (9606) EVQLVESGGDLVQPGGSLRLSCAASGFTFSSYEMNWVRQAPGKGLEWVSFISDSSSNIYYADSVKGRFTISRDNAKKSLYLQMTSLRAEDTAVYYCAREAIGSTSFDNWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPP |
Light Chain: L
Mutation: NULL
| >7MXL_L|Chain F[auth L]|REGN5714 antibody Fab fragment light chain|Homo sapiens (9606) EIVMTQSPATLSVSPGERATLSCRASQSVSSSLAWYQQKPGQAPRRLIYSASTRATGIPARFSGSGSGTEFTLTISSLQSEDFAIYYCHQYNNWPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >7MXL_C|Chain C|Major pollen allergen Bet v 1-A|Betula pendula (3505) MGVFNYETETTSVIPAARLFKAFILDGDNLFPKVAPQAISSVENIEGNGGPGTIKKISFPEGFPFKYVKDRVDEVDHTNFKYNYSVIEGGPIGDTLEKISNEIKIVATPDGGSILKISNKYHTKGDHEVKAEQVKASKEMGETLLRAVESYLLAHSDAYN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition L: SER30 SER31 SER32 ARG46 TYR49 SER50 SER52 THR53 ARG54 THR56 GLY57 TYR91 ASN92 TRP94 H: THR28 SER30 SER31 TYR32 GLU33 PHE50 SER52 ASP53 SER54 SER55 ASN57 TYR59 ARG98 GLU99 ALA100 ILE101 GLY102 SER103 THR104 C: GLY2 VAL3 PHE4 ASN5 GLU7 GLU9 ARG71 ASP73 GLU74 ASP76 ASN79 LYS81 ASN83 TYR84 SER85 GLU97 LYS98 SER100 ASN101 GLU102 LYS104 VAL106 LYS116 SER118 ASN119 LYS120 HIS122 THR123 LYS124 GLY125 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
