Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4360 |
| PDBID: | 7MXL |
| Chains: | AB_C |
| Organism: | Homo sapiens, Betula pendula |
| Method: | EM |
| Resolution (Å): | 3.20 |
| Reference: | 10.1016/j.jaci.2021.05.038 |
| Antibody | |
| Antibody: | REGN5713 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Bremzalerbart(Phase-III) |
| Antigen | |
| Antigen: | Major pollen allergen Bet v 1-A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7MXL_A|Chain A|REGN5713 antibody Fab fragment heavy chain|Homo sapiens (9606) QVQLQESGPGLVKPSETLSLTCSVSGGSITNYFWTWIRQSPGKGLEWIGYIYYSGGTNYNPSLKSRVTISIDTSKNQFSLNMNSVTAADTAVYYCAGSYYYGVDVWGQGTTVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPP |
Light Chain: B
Mutation: NULL
| >7MXL_B|Chain B|REGN5713 antibody Fab fragment light chain|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCRASQSIKSFLAWYRQKPGQAPRLLIYDASNRPTGIPARFSGSGSGTDFTLTINSLESEDFAVYFCQQRNNWPFTFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >7MXL_C|Chain C|Major pollen allergen Bet v 1-A|Betula pendula (3505) MGVFNYETETTSVIPAARLFKAFILDGDNLFPKVAPQAISSVENIEGNGGPGTIKKISFPEGFPFKYVKDRVDEVDHTNFKYNYSVIEGGPIGDTLEKISNEIKIVATPDGGSILKISNKYHTKGDHEVKAEQVKASKEMGETLLRAVESYLLAHSDAYN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: THR31 ASN32 TYR33 PHE34 TYR51 TYR53 TYR54 SER55 GLY57 ASN59 TYR100 TYR101 TYR102 B: LYS30 PHE32 TYR49 ASP50 ARG91 ASN92 ASN93 TRP94 PHE96 C: THR11 SER12 VAL13 PRO32 LYS33 VAL34 PRO36 GLN37 SER40 PRO60 GLU61 GLU142 THR143 ARG146 ALA147 GLU149 SER150 TYR151 LEU153 ALA154 HIS155 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
