Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 436 |
| PDBID: | 3BSZ |
| Chains: | LH_F |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.38 |
| Reference: | 10.1111/j.1742-4658.2008.06705.x |
| Antibody | |
| Antibody: | 3BSZ Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasma retinol-binding protein |
| Antigen mutation: | No |
| Durg Target: | P02753 |
Sequence information
Antibody
Heavy Chain: L
Mutation: NULL
| >3BSZ_L|Chain G[auth L], I[auth M]|Fab fragment heavy chain|Mus musculus (10090) DIVLTQSPSSLAVSLGQRATISCRASESVDSYGNSFMHWYQQKPGQPPKLLIYRASNLESGIPARFSGSGSRTDFTLTINPVEADDVATYYCQQSNEDPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR |
Light Chain: H
Mutation: NULL
| >3BSZ_H|Chain H, J[auth N]|Fab fragment light chain|Mus musculus (10090) DVQLQESGTVLARPGASVKMSCKASGYSFTSYWMHWIKQRPGQGLEWIGGVYPGDSHTSYNQKFKGKAKLTAVTSASTAYMELSSLTNEDSAVYYCTRSGFDYGNEDWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKI |
Antigen
Chain: F
Mutation: NULL
| >3BSZ_F|Chain E, F|Plasma retinol-binding protein|Homo sapiens (9606) ERDCRVSSFRVKENFDKARFSGTWYAMAKKDPEGLFLQDNIVAEFSVDETGQMSATAKGRVRLLNNWDVCADMVGTFTDTEDPAKFKMKYWGVASFLQKGNDDHWIVDTDYDTYAVQYSCRLLNLDGTCADSYSFVFSRDPNGLPPEAQKIVRQRQEELCLARQYRLIVHNGYCDG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR32 GLY33 ASN34 PHE36 TYR53 ARG54 ASN57 SER60 SER95 ASN96 ASP98 TYR100 H: SER28 THR30 SER31 TYR32 TYR52 GLY54 ASP55 PHE101 ASP102 TYR103 F: CYS4 ALA28 ASP31 ARG62 ASN142 GLY143 LEU144 PRO146 GLN149 LYS150 VAL152 ARG153 GLN156 GLU157 CYS160 ALA162 ARG163 GLN164 TYR165 ARG166 LEU167 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
