Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4353 |
| PDBID: | 7MWW |
| Chains: | HL_E |
| Organism: | Hepacivirus hominis, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.71 |
| Reference: | 10.1038/s41586-021-03913-5 |
| Antibody | |
| Antibody: | 2A12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | hepatitis C virus envelope full-length glycoprotein 2 (eE2) |
| Antigen mutation: | No |
| Durg Target: | P26660 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7MWW_H|Chain B[auth H]|2A12 Fab Heavy chain|Mus musculus (10090) EVQLQQSGAELVKPGASVKLSCTASGFNIKDTYIHWVNQRPEQGLEWIGRIDPANGHTQYDPKFQGKATITADTSSNTAYLQLSSLTSEDTAVYYCATSDYSYALDSWGQGTSVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPT |
Light Chain: L
Mutation: NULL
| >7MWW_L|Chain C[auth L]|2A12 Fab light chain|Mus musculus (10090) MESQTQVLMFLLLWVSGACADIVMTQSPSSLAMSVGQKVTMSCKSSQSLLNSNNQKNYLAWYQQKPGQSPKLLVYFASTRESGVPDRFIGSGSGTDFTLTISSVQAEDLADYFCQQHYSTPYTFGGGTKLEIRRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >7MWW_E|Chain A[auth E]|Core protein precursor eE2|Hepacivirus C (3052230) RTHTVGGSAAQTTGRLTSLFDMGPRQKIQLVNTNGSWHINRTALNCNDSLHTGFIASLFYTHSFNSSGCPERMSACRSIEAFRVGWGALQYEDNVTNPEDMRPYCWHYPPRQCGVVSAKTVCGPVYCFTPSPVVVGTTDRLGAPTYTWGENETDVFLLNSTRPPLGSWFGCTWMNSSGYTKTCGAPPCRTRADFNASTDLLCPTDCFRKHPDTTYLKCGSGPWLTPRCLIDYPYRLWHYPCTVNYTIFKIRMYVGGVEHRLTAACNFTRGDR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: GLU1 VAL2 GLY26 PHE27 LYS30 ASP31 THR32 TYR33 ASP52 ALA54 THR98 SER99 ASP100 TYR101 ALA104 ASP106 L: LEU52 TYR55 GLU61 SER62 E: ILE630 PHE631 LYS632 ILE633 ARG634 TYR636 VAL640 GLU641 ARG643 LEU644 THR645 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
