Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 435 |
| PDBID: | 3BQU |
| Chains: | DC_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1016/j.jmb.2008.07.057 |
| Antibody | |
| Antibody: | anti-idiotype 3H6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 2F5 Fab heavy chain |
| Antigen mutation: | No |
| Durg Target: | Q6PYX1; P01857; P01860; P01861; |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >3BQU_D|Chain D|3H6 Fab heavy chain|Mus musculus (10090) MGWSWIFLFLLSGTAGVHSGVQLQQSGPELVKPGASVKMSCKASGYSFTDYFMHWVKQSHGKSLDWIGYINCYTGATNYSQKFKGKATFTVDTSSNTAYMQFNSLTSEDSAVYYCARTSIGYGSSPPFPYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: C
Mutation: NULL
| >3BQU_C|Chain C|3H6 Fab light chain|Mus musculus (10090) MTMFSLALLLSLLLLCVSDSGAETTVTQSPASLSMSIGEKVTIRCITSTDIDDDMNWYQQKPGEPPRLLISDGNTLRPGVPSRFSSSGYGTDFVFTIENMLSEDVADYYCLQSDNLPYTFGGGTNLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR |
Antigen
Chain: B
Mutation: NULL
| >3BQU_B|Chain B|2F5 Fab' heavy chain|Homo sapiens (9606) RITLKESGPPLVKPTQTLTLTCSFSGFSLSDFGVGVGWIRQPPGKALEWLAIIYSDDDKRYSPSLNTRLTITKDTSKNQVVLVMTRVSPVDTATYFCAHRRGPTTLFGVPIARGPVNAMDVWGQGITVTISSTSTKGPSVFPLAPSSKSTAGGAAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYTCNVNHKPSNTKVDKRVEPKSC |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASP30 ASP32 ASP50 TYR67 ASP92 ASN93 D: THR30 ASP31 TYR32 PHE33 ASN52 TYR54 THR55 SER100 ILE101 GLY102 TYR103 GLY104 B: PHE32 ASP56 ASP57 ASP58 LYS59 ARG60 TYR61 PRO63 SER64 ASN66 THR67 ARG68 THR85 ARG86 PRO110 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
