Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4332 |
| PDBID: | 7MPG |
| Chains: | FG_B |
| Organism: | human respiratory syncytial virus, Human immunodeficiency virus 1, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.40 |
| Reference: | 10.1080/19420862.2021.1955812 |
| Antibody | |
| Antibody: | AM14 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | respiratory syncytial virus (RSV) Fusion glycoprotein F0 |
| Antigen mutation: | Yes |
| Durg Target: | P03420; P11209; |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >7MPG_F|Chain D, F, H|AM14 Fab Heavy Chain|Homo sapiens (9606) CVQLVESGGGVVQPGRSLRLSCAASGFSFSHYAMHWVRQAPGKGLEWVAVISYDGENTYYADSVKGRFSISRDNSKNTVSLQMNSLRPEDTALYYCARDRIVDDYYYYGMDVWGQGATVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKGSENLYFQGSHHHHHH |
Light Chain: G
Mutation: NULL
| >7MPG_G|Chain E, G, I|AM14 Fab Light Chain|Homo sapiens (9606) METPAELLFLLLLWLPDTTGDIQMTQSPSSLSASVGDRVTITCQASQDIKKYLNWYHQKPGKVPELLMHDASNLETGVPSRFSGRGSGTDFTLTISSLQPEDIGTYYCQQYDNLPPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: N101C/E136F/V153L/I236C
| >7MPG_B|Chain A, B, C|Fusion glycoprotein F0,Envelope glycoprotein|Human respiratory syncytial virus (11250) QNITEEFYQSTCSAVSKGYLSALRTGWYTSVITIELSNIKENKCNGTDAKVKLIKQELDKYKNAVTELQLLMQSTPATNNRAFLGFLLGVGSAIASGVAVCKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTFKVLDLKNYIDKQLLPILNKQSCSISNIETVIEFQQKNNRLLEITREFSVNAGVTTPVSTYMLTNSELLSLINDMPITNDQKKLMSNNVQIVRQQSYSIMCIIKEEVLAYVVQLPLYGVIDTPCWKLHTSPLCTTNTKEGSNICLTRTDRGWYCDNAGSVSFFPQAETCKVQSNRVFCDTMNSLTLPSEVNLCNVDIFNPKYDCKIMTSKTDVSSSVITSLGAIVSCYGKTKCTASNKNRGIIKTFSNGCDYVSNKGVDTVSVGNTLYYVNKQEGKSLYVKGEPIINFYDPLVFPSDEFDASISQVNEKINQSLAFIRKSDELLSAIGGYIPEAPRDGQAYVRKDGEWVLLSTFLGGLVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition F: SER30 HIS31 TYR52 ASP53 GLU55 ARG96 ILE97 ASP99 G: TYR32 ASP50 TYR91 ASN93 B: ASN426 LYS427 ASN428 ARG429 ILE432 LYS445 GLY446 LYS461 GLU463 LYS465 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
