Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4318 |
| PDBID: | 7MLV |
| Chains: | FK_D |
| Organism: | Rattus norvegicus, Sus scrofa |
| Method: | EM |
| Resolution (Å): | 4.10 |
| Reference: | 10.1038/s41586-021-04022-z |
| Antibody | |
| Antibody: | 3D1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Glycine receptor alpha 1 |
| Antigen mutation: | No |
| Durg Target: | P23415 |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >7MLV_F|Chain B[auth F], D[auth G], F[auth H], H[auth L]|3D1 Fab Heavy Chain|Rattus norvegicus (10116) QVQLQQSGAELMKPGAAVKISCKATGHTISRYWIDWLKQRPGHGLEWIGEILPGSGSTNYNEKFKGKATFTAEKSSNTAYMQLSSLTSEDSAVYYCAMGVRGNYFDYWGQGTTLTVSS |
Light Chain: K
Mutation: NULL
| >7MLV_K|Chain A[auth I], C[auth J], E[auth K], G[auth M]|3D1 Fab Light Chain|Rattus norvegicus (10116) DIVMTQSHKFMSTSVGDRVSITCKASQDVSTAVAWYQQKPGQSPKLLIYWASTRHTGVPGRFTGSGSGTDYTLTISSVQAEDLSLYYCQQHYSTPRTFGGGTKLEIK |
Antigen
Chain: D
Mutation: NULL
| >7MLV_D|Chain I[auth A], J[auth B], K[auth C], L[auth D]|Glycine receptor alpha 1|Sus scrofa (9823) MYRFNTLRLYLWETIVFFSLAASKEAEAARSASKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKSPMLNLFQEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNTTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition K: ASP28 SER30 THR31 ALA32 TRP50 TYR92 F: ARG31 TYR32 TRP33 LEU52 GLY99 VAL100 ARG101 GLY102 ASN103 D: LYS33 GLY34 PRO35 PRO36 MET163 ASN164 ASP165 ILE167 TYR197 CYS198 THR199 LYS200 HIS201 TYR202 LYS206 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
