Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4316 |
| PDBID: | 7MLU |
| Chains: | LM_A |
| Organism: | Rattus norvegicus, Sus scrofa |
| Method: | EM |
| Resolution (Å): | 4.10 |
| Reference: | 10.1038/s41586-021-04022-z |
| Antibody | |
| Antibody: | 3D1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Glycine receptor alpha 1 |
| Antigen mutation: | No |
| Durg Target: | P23415 |
Sequence information
Antibody
Heavy Chain: L
Mutation: NULL
| >7MLU_L|Chain B[auth F], D[auth G], F[auth H], H[auth L], J[auth N]|3D1 Fab Heavy Chain|Rattus norvegicus (10116) QVQLQQSGAELMKPGAAVKISCKATGHTISRYWIDWLKQRPGHGLEWIGEILPGSGSTNYNEKFKGKATFTAEKSSNTAYMQLSSLTSEDSAVYYCAMGVRGNYFDYWGQGTTLTVSS |
Light Chain: M
Mutation: NULL
| >7MLU_M|Chain A[auth I], C[auth J], E[auth K], G[auth M], I[auth O]|3D1 Fab Light Chain|Rattus norvegicus (10116) DIVMTQSHKFMSTSVGDRVSITCKASQDVSTAVAWYQQKPGQSPKLLIYWASTRHTGVPGRFTGSGSGTDYTLTISSVQAEDLSLYYCQQHYSTPRTFGGGTKLEIK |
Antigen
Chain: A
Mutation: NULL
| >7MLU_A|Chain K[auth A], L[auth B], M[auth C], N[auth D], O[auth E]|Glycine receptor alpha 1|Sus scrofa (9823) MYRFNTLRLYLWETIVFFSLAASKEAEAARSASKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCNIFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEITTDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGLTLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRRHHKSPMLNLFQEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNTTTNPPPAPSKSPEEMRKLFIQRAKKIDKISRIGFPMAFLIFNMFYWIIYKIVRREDVHNQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition M: ASP28 VAL29 SER30 THR31 TRP50 HIS91 TYR92 ARG96 L: ARG31 TYR32 TRP33 ASP35 GLU50 LEU52 SER55 SER57 GLY99 VAL100 ARG101 GLY102 ASN103 A: LYS33 GLY34 PRO35 PRO36 GLN155 MET163 ASN164 ILE167 TYR197 CYS198 THR199 LYS200 HIS201 TYR202 ASN203 GLY205 LYS206 THR208 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
