Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4312 |
| PDBID: | 7MLH |
| Chains: | ED_B |
| Organism: | Homo sapiens, Dermatophagoides pteronyssinus |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1093/pnasnexus/pgac054 |
| Antibody | |
| Antibody: | 2F10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mite allergen Der p 2.0103 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7MLH_E|Chain C, E|IgE Heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGGTFNTYSLSWVRQAPGQGLEWMGGIIPNFGRGNYAQKFQGRVTITADESSAYMELSSLRSEDTAVYYCATFRLTGYSGSGSYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHH |
Light Chain: D
Mutation: NULL
| >7MLH_D|Chain A, D|IgE Light Chain|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVTITCRASQNINNWLAWYQQKPGKAPNLLIYKASTLETGVPSRFSGSGSGTEFTLTISSLQPDDLATYYCQQYHSHRTFGQGTKVEVKGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: B
Mutation: NULL
| >7MLH_B|Chain B, F|Der p 2 variant 3|Dermatophagoides pteronyssinus (6956) DQVDVKDCANHEIKKVLVPGCHGSEPCIIHRGKPFQLEALFEANQNSKTAKIEIKASIDGLEVDVPGIDPNACHYMKCPLVKGQQYDIKYTWNVPKIAPKSENVVVTVKVMGDNGVLACAIATHAKIRD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition D: TRP32 TYR91 HIS92 SER93 HIS94 ARG95 E: SER33 TRP47 GLY50 ILE51 ILE52 PHE55 GLY56 ARG57 ASN59 LEU99 THR100 GLY101 TYR102 B: ARG31 ILE58 ASP59 GLY60 LEU61 GLU62 VAL63 ASP64 ILE97 ALA98 PRO99 LYS100 SER101 GLU102 ASN103 VAL105 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
