Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4308 |
| PDBID: | 7MJS |
| Chains: | HL_X |
| Organism: | synthetic construct, Gallus gallus |
| Method: | EM |
| Resolution (Å): | 3.03 |
| Reference: | 10.1038/s41586-021-03650-9 |
| Antibody | |
| Antibody: | 2AG3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Major Facilitator Superfamily Domain containing 2A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7MJS_H|Chain A[auth H]|2AG3 Fab heavy chain|Synthetic construct (32630) EISEVQLVESGGGLVQPGGSLRLSCAASGFNIYSSSIHWVRQAPGKGLEWVAYIYSYSGYTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSLEYLYSSGYQYKWATGLDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >7MJS_L|Chain B[auth L]|2AG3 Fab light chain|Synthetic construct (32630) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSFYNQPFTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: X
Mutation: NULL
| >7MJS_X|Chain C[auth X]|Major Facilitator Superfamily Domain containing 2A|Gallus gallus (9031) MAGGGGAERVRVGAAAAGLLPPSCRQPRRRESRERLSVCSKLCYAVGGAPYQTTGCALGFFLQIYLLDVAQLDPFYASIILFVGRAWDAITDPMVGFFISKTPWTRFGRLMPWIIFSTPFAVISYFLIWFVPDISTGQVMWYLIFYCIFQTLVTCFHVPYSALTMFISREQSERDSATAYRMTVEVLGTVLGTAIQGQIVGKAVTPCIENPPFLSETNFSVAIRNVNMTHYTGSLADTRNAYMVAAGVIGGLYILCAVILSVGVREKRESSELQSDEPVSFFRGLKLVMNHGAYIKLITGFLFTSLAFMLLEGNFALFCTYTLGFRNEFQNILLAIMLSATLTIPFWQWFLTRFGKKTAVYVGISSAVPFLITVVVLDSNLVVTYIVAVAAGISVAAAFLLPWSMLPDVIDDFKLQHPESRGHEAIFFSFYVFFTKFTSGVSLGISTLSLDFAGYQTRGCSQPSEVNITLKLLVSAVPVGLILLGLLLFKLYPIDEEKRRENKKALQDLREESNSSSESDSTELANIVENLYFQGSHHHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: SER34 SER35 TYR53 TYR55 TYR57 SER58 TYR60 LEU103 GLU104 TYR105 LEU106 TYR111 GLN112 TYR113 LYS114 TRP115 L: TYR50 PHE94 TYR95 GLN97 X: VAL204 THR205 PRO206 CYS207 ILE208 GLU209 PRO211 PRO212 PHE213 LEU214 SER215 GLU216 VAL221 ALA222 ILE223 ASN225 VAL226 ASN227 MET228 HIS230 TYR231 SER234 GLY324 ARG326 ASN327 CYS460 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
