Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4296 |
| PDBID: | 7MHY |
| Chains: | OP_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.70 |
| Reference: | 10.1126/science.abg4998 |
| Antibody | |
| Antibody: | 3H02 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Protein-cysteine N-palmitoyltransferase HHAT |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: O
Mutation: NULL
| >7MHY_O|Chain D[auth O]|3H02 Fab heavy chain|Mus musculus (10090) MDWLWNLLFLMAAAQSAQAQIQLVQSGPELKKPGETVKISCKASGYTFTNYGMNWVRQAPGKGLKWMGWINTYTGEPTYADDFKGRFAFSLETSASTAYLQINNLKDEDMATYFCARVWNYDYYFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSS |
Light Chain: P
Mutation: NULL
| >7MHY_P|Chain E[auth P]|3H02 Fab light chain|Mus musculus (10090) MKLPVRLLVLMFWIPASRSDVLMTQTPLSLPVSLGDQVSISCRSSQSIVHSNGNTYLEWYLQKPGQSPKLLIYRVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSHVPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTK |
Antigen
Chain: A
Mutation: NULL
| >7MHY_A|Chain A|Protein-cysteine N-palmitoyltransferase HHAT|Homo sapiens (9606) MLPRWELALYLLASLGFHFYSFYEVYKVSREHEEELDQEFELETDTLFGGLKKDATDFEWSFWMEWGKQWLVWLLLGHMVVSQMATLLARKHRPWILMLYGMWACWCVLGTPGVAMVLLHTTISFCVAQFRSQLLTWLCSLLLLSTLRLQGVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCWQQLPAASTSYSFPWMLAYVFYYPVLHNGPILSFSEFIKQMQQQEHDSLKASLCVLALGLGRLLCWWWLAELMAHLMYMHAIYSSIPLLETVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPALPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPVGGSQHGLLGTLFSTAMTFAFVSYWHGGYDYLWCWAALNWLGVTVENGVRRLVETPCIQDSLARYFSPQARRRFHAALASCSTSMLILSNLVFLGGNEVGKTYWNRIFIQGWPWVTLSVLGFLYCYSHVGIAWAQTYATD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition O: THR49 ASN50 TYR51 GLY52 TRP69 ASN71 THR72 TYR73 THR74 VAL118 TRP119 ASN120 TYR121 ASP122 TYR123 TYR124 P: HIS50 SER51 ASN52 ASN54 TYR56 GLY115 TRP120 A: ARG131 SER132 GLN133 CYS188 TRP189 GLN190 GLN191 LEU192 PRO193 ALA194 SER196 THR197 GLN230 ILE352 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
