Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4295 |
| PDBID: | 7MHY |
| Chains: | MN_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.70 |
| Reference: | 10.1126/science.abg4998 |
| Antibody | |
| Antibody: | 1C06 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Protein-cysteine N-palmitoyltransferase HHAT |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >7MHY_M|Chain B[auth M]|1C06 Fab heavy chain|Mus musculus (10090) MERHWIFLFLFSVTAGVHSQVQLRQSGAELAKPGASVKMSCKASGYTFTNYWLHWIKQRPGQGLEWIGYISPNTGSTEYSQKFKDRATLTADKSSSTAYMQLSSLTSEDSAVYYCAGSRITGTWFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSS |
Light Chain: N
Mutation: NULL
| >7MHY_N|Chain C[auth N]|1C06 Fab light chain|Mus musculus (10090) MKLPVRLLVLMFWIPASRSDVLMTQTPLSLPVSLGDQVSISCRSSQSIVHSNGNTYLEWYLQKPGQSPKLLIYWVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSHVPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTK |
Antigen
Chain: A
Mutation: NULL
| >7MHY_A|Chain A|Protein-cysteine N-palmitoyltransferase HHAT|Homo sapiens (9606) MLPRWELALYLLASLGFHFYSFYEVYKVSREHEEELDQEFELETDTLFGGLKKDATDFEWSFWMEWGKQWLVWLLLGHMVVSQMATLLARKHRPWILMLYGMWACWCVLGTPGVAMVLLHTTISFCVAQFRSQLLTWLCSLLLLSTLRLQGVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCWQQLPAASTSYSFPWMLAYVFYYPVLHNGPILSFSEFIKQMQQQEHDSLKASLCVLALGLGRLLCWWWLAELMAHLMYMHAIYSSIPLLETVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPALPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPVGGSQHGLLGTLFSTAMTFAFVSYWHGGYDYLWCWAALNWLGVTVENGVRRLVETPCIQDSLARYFSPQARRRFHAALASCSTSMLILSNLVFLGGNEVGKTYWNRIFIQGWPWVTLSVLGFLYCYSHVGIAWAQTYATD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition M: THR47 PHE48 THR49 ASN50 TRP52 THR74 SER76 ARG119 ILE120 THR121 GLY122 TRP124 TYR127 N: SER47 VAL49 HIS50 ASN54 TYR56 TYR73 TRP74 ASN77 GLY90 SER91 GLY92 THR93 SER116 A: HIS32 GLU35 LEU36 GLN38 GLU39 PHE40 GLU41 THR56 GLN150 GLU154 ARG157 GLU163 TYR167 SER272 SER273 ILE274 PRO275 LEU276 GLU278 THR279 GLY448 GLU451 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
