Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4292 |
| PDBID: | 7MEP |
| Chains: | MJ_F |
| Organism: | Macaca mulatta, Human immunodeficiency virus |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1126/sciadv.abk2039 |
| Antibody | |
| Antibody: | RM19R Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 SOSIP.v5.2(7S) envelope glycoprotein - gp120 |
| Antigen mutation: | No |
| Durg Target: | P00750 |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >7MEP_M|Chain B[auth I], H[auth M], L[auth N]|RM19R mAb Heavy chain|Macaca mulatta (9544) EVQLVESGPGLVRPSETLSLTCAVSGDSISTNNGWSWIRQTPGKGLEWIGYINGRSGSTRYNPSLQSRVTISTDTSGNQFSLKVNSVTAADTAKYYCAFFWSTYYKRFDVWGPGVRVTVSS |
Light Chain: J
Mutation: NULL
| >7MEP_J|Chain A[auth G], G[auth J], K|RM19R mAb Light chain|Macaca mulatta (9544) AIRMTQSPAILSLSPGERATLSCRASQSVDSRLAWYQQKPGQSPRLLIYDVSSRATGIPDRFSGSGSGTEFTLTISSLEPEDVAVYFCHQENDWPWTFGQGTKVEIK |
Antigen
Chain: F
Mutation: NULL
| >7MEP_F|Chain C[auth A], D[auth B], I[auth C], J[auth D], M[auth E], N[auth F]|BG505 SOSIPv5.2(7S) - gp120|Human immunodeficiency virus (12721) MKRGLCCVLLLCGAVFVSPSQEIHARFRRGARAENLWVTVYYGVPVWKDAETTLFCASDAKAYETKKHNVWATHCCVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVIIRSENITNNAKNILVQLNESVQINCTRPNNNTVKSIRIGPGQWFYYTGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGRRRRRRAVGIGAVSLGFLGAAGSTMGAASMTLTVQARNLLSGIVQQQSNLLRAPECQQHLLKDTHWGIKQLQARVLAVEHYLRDQQLLGIWGCSGKLICCTNVPWNSSWSNRNLSEIWDNMTWLQWDKEISNYTQIIYGLLEESQNQQEKNEQDLLELD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition J: ARG32 ASN92 ASP93 TRP94 TRP96 M: ASN33 GLY34 TYR50 ARG53 SER54 SER56 THR57 ARG58 PHE95 SER97 THR98 TYR99 F: LYS655 GLU657 GLN658 ASP659 LEU660 LEU661 GLU662 LEU663 ASP664 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
