Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4290 |
| PDBID: | 7MEP |
| Chains: | HL_A |
| Organism: | Macaca mulatta, Human immunodeficiency virus |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1126/sciadv.abk2039 |
| Antibody | |
| Antibody: | Rh.33172 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 SOSIP.v5.2(7S) envelope glycoprotein - gp120 |
| Antigen mutation: | No |
| Durg Target: | P00750 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7MEP_H|Chain E[auth H]|Rh.33172 mAb.1 Heavy chain|Macaca mulatta (9544) QVQLVQSGAEVKMPGTSVKLSCKTSGYTFTSYNINWVRQAPGQALEWMGWINPNNGTTDYAQKFQGRVTMTRDTSTTTAYMQLNSLRSEDTAVYYCARARGGYEDDDGYHYTGYGLDSWGQGVVVTVSS |
Light Chain: L
Mutation: NULL
| >7MEP_L|Chain F[auth L]|Rh.33172 mAb.1 Light chain|Macaca mulatta (9544) DIQMTQSPSSLSASIGDRVTVTCRASQGINMQLCWYQLKPGKAPTLLIYGTSGLQTGVSSRFSGSGSGTNFTLTISSLQPEDVATYYCQQDYTTPFTFGPGTKLDIK |
Antigen
Chain: A
Mutation: NULL
| >7MEP_A|Chain C[auth A], D[auth B], I[auth C], J[auth D], M[auth E], N[auth F]|BG505 SOSIPv5.2(7S) - gp120|Human immunodeficiency virus (12721) MKRGLCCVLLLCGAVFVSPSQEIHARFRRGARAENLWVTVYYGVPVWKDAETTLFCASDAKAYETKKHNVWATHCCVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCTNVSTVQCTHGIKPVVSTQLLLNGSLAEEEVIIRSENITNNAKNILVQLNESVQINCTRPNNNTVKSIRIGPGQWFYYTGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGRRRRRRAVGIGAVSLGFLGAAGSTMGAASMTLTVQARNLLSGIVQQQSNLLRAPECQQHLLKDTHWGIKQLQARVLAVEHYLRDQQLLGIWGCSGKLICCTNVPWNSSWSNRNLSEIWDNMTWLQWDKEISNYTQIIYGLLEESQNQQEKNEQDLLELD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: GLY26 TYR27 THR28 PHE29 THR30 SER31 TYR32 ASN53 ASN54 THR73 SER74 ARG94 ARG96 GLY97 GLY98 TYR99 L: TYR49 LEU54 GLN55 THR56 GLY57 A: ASN137 LYS168 LYS169 GLN170 LYS171 VAL172 TYR173 PRO206 LYS207 ASN301 THR303 VAL304 LYS305 SER306 ILE307 ARG308 GLY314 TRP316 TYR318 TYR319 GLY321 ASP322 ILE323 GLY324 ASP325 GLN440 GLY441 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
