Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 429 |
| PDBID: | 3BDY |
| Chains: | HL_V |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1126/science.1165480 |
| Antibody | |
| Antibody: | bH1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Vascular endothelial growth factor A(VEGF-A) |
| Antigen mutation: | No |
| Durg Target: | P15692 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3BDY_H|Chain A[auth H]|Fab Fragment -Heavy Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: L
Mutation: NULL
| >3BDY_L|Chain B[auth L]|Fab Fragment -Light Chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDIPRSISGYVAWYQQKPGKAPKLLIYWGSYLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: V
Mutation: NULL
| >3BDY_V|Chain C[auth V]|Vascular endothelial growth factor A|Homo sapiens (9606) GQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR33 ARG50 ASN54 TYR56 TRP95 ASP98 GLY99 TYR100 L: ARG27 SER28 ILE29 SER30 GLY31 TYR32 TRP50 TYR53 HIS91 TYR92 THR93 THR94 V: TYR45 LYS48 GLN79 MET81 ARG82 ILE83 LYS84 PRO85 HIS86 GLN87 GLY88 GLN89 HIS90 ILE91 GLY92 GLU93 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
