Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4280 |
| PDBID: | 7M8L |
| Chains: | GJ_A |
| Organism: | Ebola virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.90 |
| Reference: | 10.1016/j.cell.2021.09.035 |
| Antibody | |
| Antibody: | rEBOV-442 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus Virion spike glycoprotein 1 |
| Antigen mutation: | No |
| Durg Target: | Q05320; P00750; |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >7M8L_G|Chain B[auth G], H, N[auth I]|rEBOV-442 Fab heavy chain|Homo sapiens (9606) EVQLVESGGGLVKPGGSLRLSCTGSGGTGFTFKYAGMSWVRQAPGKGPEWIGRIKSRIDGGTTDYAAPVKDRFIVSRDDSRNTLYLQMNSLKTEDTAVYYCATGSGKGPSASFGESYYYYDFINVWGKGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: J
Mutation: NULL
| >7M8L_J|Chain C[auth J], I[auth K], O[auth L]|rEBOV-442 Fab light chain|Homo sapiens (9606) ESVLTQSPGTLSLSPGERATLSCRASQSISRKYLAWYQQKPGQAPRLLIYGSSSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCHQYESSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLRSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7M8L_A|Chain A, G[auth B], M[auth C]|Virion spike glycoprotein 1|Ebola virus (1570291) MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSVTKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYASGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition G: THR28 PHE32 LYS33 ARG57 ILE58 ASP59 GLY60 THR62 SER80 PRO109 SER110 ALA111 SER112 PHE113 GLY114 GLU115 SER116 TYR117 TYR118 TYR119 TYR120 ASP121 PHE122 J: LYS32 TYR50 A: PRO116 THR144 GLN221 THR223 GLU229 GLU231 LEU233 VAL242 GLN243 LEU256 THR259 ILE260 LYS265 ARG266 SER267 ASN268 THR269 THR270 GLY271 LYS272 LEU273 ILE274 TRP275 LYS276 VAL277 ASN278 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
