Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 428 |
| PDBID: | 3B9K |
| Chains: | DC_F |
| Organism: | Rattus rattus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.jmb.2008.09.069 |
| Antibody | |
| Antibody: | YTS156.7.7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | T cell surface glycoprotein CD8 beta chain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >3B9K_D|Chain B[auth D], F[auth H]|Fab Heavy chain|rattus rattus (10117) QVRLQESGPGLVQPSQTLSLTCSVSGFSLISDSVHWVRQPPGKGLEWMGGIWADGSTEYNSALKSRLSISRDTSKSQGFLKMNSLQTDDTAIYFCTSNRESYYFDYWGQGTMVTVSSAQTTAPSVYPLAPGCGDTTSSTVTLGCLVKGYFPEPVTVTWNSGALSSDVHTFPAVLQSGLYTLTSSVTSSTWPSQTVTCNVAHPASSTKVDKAVEA |
Light Chain: C
Mutation: NULL
| >3B9K_C|Chain A[auth C], E[auth L]|Fab Light Chain|rattus rattus (10117) DIKMTQSPASLSASLGDKVTITCQASQNIDKYIAWYQQKPGKAPRQLIHYTSTLVSGTPSRFSGSGSGRDYTFSISSVESEDIASYYCLQYDTLYTFGAGTKLELKRADAAPTVSIFPPSMEQLTSGGATVVCFVNNFYPRDISVKWKIDGSEQRDGVLDSVTDQDSKDSTYSMSSTLSLTKVEYERHNLYTCEVVHKTSSSPVVKSFNRGEC |
Antigen
Chain: F
Mutation: NULL
| >3B9K_F|Chain D[auth B], H[auth F]|T-cell surface glycoprotein CD8 beta chain|Mus musculus (10090) LIQTPSSLLVQTNHTAKMSCEVKSISKLTSIYWLRERQDPKDKYFEFLASWSSSKGVLYGESVDKKRNIILESSDSRRPFLSIMNVKPEDSDFYFCATVGSPKMVFGTGTKLTVVDVSSADLVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASP30 TYR32 HIS49 TYR50 TYR91 ASP92 THR93 D: ILE30 SER31 ASP32 SER33 TRP52 ALA53 ASP54 SER56 GLU58 ASN95 ARG96 GLU97 SER98 TYR99 F: GLU21 VAL22 LYS23 SER24 ILE25 SER26 LYS27 LEU28 TRP51 SER52 SER53 SER54 LYS55 GLY56 VAL57 LEU71 GLU72 SER73 SER74 ASP75 SER76 ARG77 ARG78 PHE80 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
