Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4254 |
| PDBID: | 7M30 |
| Chains: | MN_C |
| Organism: | Human betaherpesvirus 5, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.81 |
| Reference: | 10.1126/sciadv.abm2546 |
| Antibody | |
| Antibody: | 1-103 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human cytomegalovirus (HCMV) Envelope protein UL128 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >7M30_M|Chain I[auth M]|1-103 Fab Heavy Chain|Homo sapiens (9606) QLQLQESGPGLVKPSETLSLTCSVSGDAISGSNYYWGWIRQPPGKGLQWIGSIYHTGSTFYNPSFSSRVTLSVDTSKNQFSLKLISVNAADTAVYYCARRIRGYSGTYDWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKGLEVLFQ |
Light Chain: N
Mutation: NULL
| >7M30_N|Chain J[auth N]|1-103 Fab Light Chain|Homo sapiens (9606) DIQLTQSPSFLSASVGDRVTITCRASQDISSYVAWYQQKPGNAPKLLISSASTLPSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQLNNFGPGTTVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >7M30_C|Chain B[auth C]|Envelope protein UL128|Human cytomegalovirus (10359) EECCEFINVNHPPERCYDFKMCNRFTVALRCPDGEVCYSPEKTAEIRGIVTTMTHSLTRQVVHNKLTSCNYNPLYLEADGRIRCGKVNDKAQYLLGAAGSVPYRWINLEYDKITRIVGLDQYLESVKKHKRLDVCRAKMGYMLQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition M: TRP47 SER50 TYR52 THR54 SER56 THR57 PHE58 TYR59 ASN60 PRO61 SER64 ARG95 ARG97 GLY98 TYR99 N: ASP1 ILE2 GLN27 ASP28 ILE29 SER30 TYR32 GLN90 LEU91 ASN96 ASN97 C: GLU29 CYS30 CYS31 GLU32 PHE33 ILE34 ASN35 VAL36 ASN37 HIS38 PRO40 ARG42 CYS49 ASN50 PHE52 THR53 PRO59 ASP60 GLY61 GLU62 VAL63 SER66 GLU68 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
