Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4188 |
| PDBID: | 7LR4 |
| Chains: | AB_C |
| Organism: | Mus musculus, Plasmodium berghei |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.7554/eLife.74707 |
| Antibody | |
| Antibody: | D3_2/1.12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium berghei domain 3 of hapless 2 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7LR4_A|Chain A, C[auth H]|D3_2/1.12 Fab heavy chain|Mus musculus (10090) EFQLLQSGPELVKPGASVKISCKASDYSLSDYNMNWVRQRSGKSLEWIGVINPNHGTTHYNQKFKGKATLTVDQSSSTAYMQLTSLTSEDSAVYYCASPIHYGNHVPFDYWGQGTTVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGGS |
Light Chain: B
Mutation: NULL
| >7LR4_B|Chain B, D[auth L]|D3_2/1.12 Fab light chain|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCRTGQDISNYLNWYQQKPDGTVKLLIYFTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGITLPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: N15T/S32N/N38Q
| >7LR4_C|Chain E[auth C], F[auth D]|Hapless 2|Plasmodium berghei (5821) ATITHVTIPNDCASTNSNSNECVLIIHVWNNNKFVGSQFSCSIACTNKETDQLASHINPIAPVRAFIGPNKNYAFYFIIKFLINKEITTLCKAIVKDSNGKECSIEEFELQSKESVHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: ASN54 HIS55 HIS59 TYR60 ILE100 HIS101 TYR102 GLY103 ASN104 HIS105 VAL106 B: SER30 TYR32 PHE50 ARG53 GLY91 ILE92 THR93 LEU94 TRP96 C: HIS506 THR508 ASP512 GLU522 CYS523 VAL524 ILE526 HIS528 ALA562 PRO563 VAL564 ARG565 PHE567 ASN573 TYR574 ALA575 TYR577 ILE579 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
