Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4186 |
| PDBID: | 7LR3 |
| Chains: | AB_C |
| Organism: | Mus musculus, Plasmodium berghei |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.7554/eLife.74707 |
| Antibody | |
| Antibody: | D3_2/6.14 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium berghei domain 3 of hapless 2 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7LR3_A|Chain B[auth A], E[auth H]|D3_2/6.14 Fab heavy chain|Mus musculus (10090) EVQLVESGGGLVKPGGSLKLSCAASEFTFSDYGMHWVRQAPEKGLEWVASISSGSNSIYYSDTVKGRFTISRDNAKSILFLQMTSLRSEDTAMYYCSREAYFAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPTWSETVTCNVAHPASSTKVDKKIVPRDCGGSHHHHHH |
Light Chain: B
Mutation: NULL
| >7LR3_B|Chain A[auth B], F[auth L]|D3_2/6.14 Fab light chain|Mus musculus (10090) DIQMTQSPSSLSAILGGKVTITCKASQDIHKYIAWYQHKPGKGPRLLIYYTSTLQPGIPSRFSGSGSGRDYSFSISNLEPEDVATYFCLQYDNLYTFGGGTKVEIERADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: N15T/S32N/N38Q
| >7LR3_C|Chain D[auth C]|Hapless 2|Plasmodium berghei (5821) ATITHVTIPNDCASTNSNSNECVLIIHVWNNNKFVGSQFSCSIACTNKETDQLASHINPIAPVRAFIGPNKNYAFYFIIKFLINKEITTLCKAIVKDSNGKECSIEEFELQSKESVHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: SER30 ASP31 TYR32 GLY33 SER52 SER53 GLY54 ASN56 SER57 TYR59 GLU99 ALA100 TYR101 PHE102 B: TYR32 TYR50 TYR91 ASP92 ASN93 TYR95 C: GLN539 PHE540 SER541 SER543 ALA545 ILE561 ALA562 PRO563 ARG565 LYS593 ILE595 LYS597 ASP598 SER599 ASN600 GLY601 LYS602 GLU603 ILE606 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
