Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4174 |
| PDBID: | 7LPN |
| Chains: | I_F |
| Organism: | Human immunodeficiency virus 1, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.61 |
| Reference: | 10.1016/j.str.2022.03.012 |
| Antibody | |
| Antibody: | llama J3 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 DS-SOSIP.664 envelope glycoprotein - gp160 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: I
Mutation: NULL
| >7LPN_I|Chain C[auth D], F[auth H], I|J3 VHH|Lama glama (9844) EVQLVESGGGLVQAGGFLRLSCELRGSIFNQYAMAWFRQAPGKEREFVAGMGAVPHYGEFVKGRFTISRDNAKSTVYLQMSSLKPEDTAIYFCARSKSTYISYNSNGYDYWGRGTQVTVSSAAAHHHHHH |
Antigen
Chain: F
Mutation: NULL
| >7LPN_F|Chain B[auth C], E[auth F], H[auth G]|Envelope glycoprotein gp160|Human immunodeficiency virus 1 (11676) AENLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSACTQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQCMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition I: ASN30 GLN31 TYR32 GLY52 ALA55 VAL56 HIS58 ARG71 ASN73 LYS96 SER97 THR98 TYR99 TYR100 ASP101 F: HIS105 THR106 ILE109 ILE194 THR198 THR257 ASN280 ALA281 LYS282 ASN283 SER365 GLY366 GLY367 ASP368 LEU369 GLU370 VAL371 ASN425 TRP427 GLN428 ARG429 ILE430 THR455 ARG456 ASP457 GLY458 ARG469 GLY473 ASP474 MET475 ARG476 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
