Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4165 |
| PDBID: | 7LO8 |
| Chains: | HL_Z |
| Organism: | Staphylococcus aureus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.16 |
| Reference: | 10.1038/s41589-022-00994-9 |
| Antibody | |
| Antibody: | Fab36 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Quinolone resistance protein NorA |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7LO8_H|Chain B[auth H]|Fab36 Heavy Chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAEISEVQLVESGGGLVQPGGSLRLSCAASGFTFSSSSIHWVRQAPGKGLEWVASISSSSGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARYSRYYYYAWRVGGYWGGLDYWGQGTLVTVFNQIKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >7LO8_L|Chain C[auth L]|Fab36 Light Chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYASDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSSSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: Z
Mutation: NULL
| >7LO8_Z|Chain A[auth Z]|Quinolone resistance protein NorA|Staphylococcus aureus (1280) MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLVAAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGGMSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFYFAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGLSAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSLLYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNSTFTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: GLU27 THR54 PHE55 SER56 SER57 SER58 SER59 SER78 SER80 SER81 SER83 TYR125 ARG127 TYR128 TYR129 TYR130 TYR131 ALA132 TRP133 ARG134 VAL135 GLY136 GLY137 TYR138 TRP139 ASP143 TYR144 L: GLN51 SER52 VAL53 SER54 SER55 LEU71 ILE72 TYR73 ALA75 SER76 SER77 LEU78 TYR79 GLY81 VAL82 PRO83 SER84 ARG85 PHE86 ARG90 GLU105 ASP106 GLN114 SER115 SER116 SER117 SER118 Z: ILE19 VAL22 ILE23 PRO24 VAL25 LEU26 PRO27 VAL28 LEU30 LYS31 ASP32 THR36 GLY37 SER38 LEU40 GLY41 LEU42 VAL44 ALA45 PHE47 LEU49 ARG98 ASN137 PHE140 ILE141 PRO144 GLY147 GLY148 ALA151 GLU152 HIS155 SER219 GLU222 THR223 LEU224 TYR225 SER226 LEU227 ALA230 ASP231 ASN234 TYR235 SER236 PRO237 LYS238 ILE240 SER241 ILE244 GLY247 GLY248 PHE303 ASP307 ARG310 ASN340 PHE341 PRO344 LEU345 GLY348 ALA349 LEU350 PHE351 ASP352 VAL353 HIS354 ILE355 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
