Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4164 |
| PDBID: | 7LO7 |
| Chains: | HL_Z |
| Organism: | Staphylococcus aureus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.74 |
| Reference: | 10.1038/s41589-022-00994-9 |
| Antibody | |
| Antibody: | Fab25 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Quinolone resistance protein NorA |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7LO7_H|Chain B[auth H]|Fab25 Heavy Chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAEISEVQLVESGGGLVQPGGSLRLSCAASGFTFSSSSIHWVRQAPGKGLEWVASISSSSGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARWETGYYPYWRMYGFYWALDYWGQGTLVTVFNQIKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: L
Mutation: NULL
| >7LO7_L|Chain C[auth L]|Fab25 Light Chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYASDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSSSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: Z
Mutation: NULL
| >7LO7_Z|Chain A[auth Z]|Quinolone resistance protein NorA|Staphylococcus aureus (1280) MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLVAAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGGMSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFYFAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGLSAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSLLYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNSTFTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: GLU24 ILE25 SER26 PHE53 THR54 SER57 ARG124 GLU126 TYR129 TYR130 PRO131 TYR132 TRP133 ARG134 MET135 TYR136 GLY137 PHE138 TYR139 TRP140 ALA141 LEU142 TYR144 L: VAL53 SER54 SER55 TYR73 TYR79 SER80 ARG90 Z: ILE19 GLY20 VAL22 ILE23 LEU26 PRO27 VAL28 LEU30 LYS31 ASP32 GLY34 LEU35 THR36 GLY37 SER38 ASP39 LEU40 VAL44 ARG98 ASN137 PHE140 ILE141 PRO144 GLU222 THR223 TYR225 SER226 LEU227 ALA230 ASP231 ASN234 PRO237 ILE240 SER241 ILE244 THR245 GLY247 GLY248 PHE303 ASP307 ASN340 PRO344 PHE351 ASP352 ILE355 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
