Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4154 |
| PDBID: | 7LL2 |
| Chains: | KM_E |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.73 |
| Reference: | 10.1016/j.celrep.2021.109922 |
| Antibody | |
| Antibody: | VRC33.01 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 BG505 DS-SOSIP gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: K
Mutation: NULL
| >7LL2_K|Chain C[auth H], G[auth I], K|VRC33.01 Fab Heavy chain|Homo sapiens (9606) QVQLQQWGAGLLKPSETLSLTCALYGRSLNGNYWSWIRQSPGKGLEWIGEINHSGSTYFNPSFKSRVAMSVDTSKSQFSLKLNSVTAADTGIYFCARGKRYSASYSNYFGVWGQGTQVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: M
Mutation: NULL
| >7LL2_M|Chain D[auth J], H[auth L], L[auth M]|VRC33.01 Fab Light chain|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVDITCRASQSISRWLAWYQQKPGKAPKVLIYEASLLANGVPSRFSGHFNGRESATDFTLTISSLQPDDVATYYCQHYMADPRFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >7LL2_E|Chain A, E[auth C], I[auth E]|Envelope glycoprotein gp120|Human immunodeficiency virus 1 (11676) AENLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSACTQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQCMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition K: ARG27 ASN32 ARG94 GLY95 ARG97 TYR98 SER99 ALA100 SER101 TYR102 SER103 TYR105 GLY107 M: ARG31 TRP32 TYR49 GLU50 SER52 LEU53 GLU66 E: THR278 ASN279 ASN280 ALA281 SER365 GLY366 GLY367 ASP368 GLU370 VAL371 GLN428 THR455 ARG456 ASP457 GLY458 GLY459 SER460 THR461 ASN462 SER463 THR465 GLU466 ARG469 ASP474 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
