Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4148 |
| PDBID: | 7LKH |
| Chains: | HL_A |
| Organism: | Gallus gallus, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1016/j.cell.2021.05.019 |
| Antibody | |
| Antibody: | 4G10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Sterol regulatory element-binding protein cleavage-activating protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7LKH_H|Chain B[auth H]|4G10 Fab heavy chain|Mus musculus (10090) TGVHSEVQLQQSGAELVRPGASVKLSCTASGFKIKDDYIHWVKQRPEQGLEWIGRIDPANGHTRYAPKFQDKATITADTSSNTAYLQLSSLTSEDTAVYYCTRYNDYDAFYFDYWGQGTTLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKT |
Light Chain: L
Mutation: NULL
| >7LKH_L|Chain C[auth L]|4G10 Fab kappa chain|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCRASQDIRNYLNWYQQKPDGTVKLLIYYTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQTNTLPWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: D430V
| >7LKH_A|Chain A|Sterol regulatory element-binding protein cleavage-activating protein|Gallus gallus (9031) PAMTLTEKLRERISRAFYNHGLLCASYPIPIILFTGLCILACCYPLLKLPLPGTGPVEFSTPVKDYFPPSPDVVSQQGDLSERPDWYVGAPVAYIQQIFVKATVSPWQKNFLAVDVFRSPLSRVFQLVEEIRNHALRDSSGVKSLEEVCLQVTDLLPGLKKLRNLLPEHGCLLLSPGNFWQNDRERFNADPDIIKTIHQHEPKALQTSATLKDLLFGLPGKYSGVNLYNRKRVVSYTVTLGLQRYDSRFLSSLRSRLKLLHPSPNCTLREDSIVHVHFKEEIGIAELIPLVTTYIILFAYIYFSTRKIDMVKSKWGLALAAVVTVLSSLLMSVGLCTLFGLTPTLNGGEIFPYLVVVIGLENVLVLTKSVVSTPVDLEVKLRIAQGLSNESWSIMKNMATELGIILIGYFTLVPAIQEFCLFAVVGLVSVFFLQMLFFTTVLSIDIRRMELADLNKRLPAEACLPPAKPASRSQRYERQPAVRPATPHTITLQPSSFRNLRLPKRLRVIYFFARTRLAQRLIMAGTVIWIGILVYTDPAGLRTYLTSQVTEQSPLGEAGLPPMPVPGGVLPAGDPKIDLSVFPSDPIQLSENQTQQREQQAGLEPLGRLETNQHSWAQGPEGRGNGQTELGTEAEVTWGAEDEEIWRKLSFRHWPSLFSYYNITLAKRYISILPAIPVTLYLNPQEALEVRHPQEANRYHPFLSSSGGKLNAEAQPDQTSSRLQGHRDVTLYKVAALGLASGILLVLLLFCLYRLLCPKNYGQNGLSHSRRRRGDLPCDDYGYSPPETEIVPLVLRGHLMDIECLASDGMLLVSCCLVGQIRVWDAQTGDCLTVIPKPRLRRDSSGIFDYQESWDHSPDGKTGLDDSFESSHQLKRMLSPPQPPLFCDQPDLTSLIDTNFSEQVKVAESEPRLRAVGGRQKEAGYDFSSLVGKVYEEHSTSNCMNFGGLSAPHGQAGFCVGGSTARSLGCGSEEGGCGGRRRSLGDESLSGFDKSSPLPSWGGDFESSVWSLDLQGNLIVAGRSNGKLEVWDAIEGTLRSSNDESQSGITALVFLNNRIVAARLNGSLDFFSLETHTSLNHLQFRGAPSRSSIPSSPLFSSSDVIVCQLTHTVSCAHQKPITALKAAAGRLVTGSQDHTLRVFRLEDSCCLFTLQGHSGAITAVYIDQTMVLASGGQDGAICLWDVLTGSKVSHMYAHRGDVTSLTCTTSCVISSGLDDVISIWDRSSGIKLYSIQQEMGCGSSLGVISDNLLVTGGQGCVSFWDIGYGDLLQTVYLGKSNESQPARQILVLENAAIVCNFGSELSLVYVPSVLEKLDDYKDDDDKGSDYKDDDDKGSDYKDDDDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: LYS30 ASP31 ASP32 TYR33 TRP47 ARG50 ASP52 ALA54 ASN55 HIS57 ARG59 TYR99 ASP101 TYR102 ASP103 ALA104 PHE105 L: ARG30 TYR32 TYR49 TYR50 ARG53 THR91 ASN92 LEU94 TRP96 A: SER58 THR59 HIS132 LEU134 ARG135 ASP136 SER137 SER138 GLY139 ARG246 SER249 SER250 SER253 ARG254 LEU257 LEU258 PRO260 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
