Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4145 |
| PDBID: | 7LK4 |
| Chains: | FE_P |
| Organism: | Homo sapiens, Rattus norvegicus |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1038/s41418-022-00961-w |
| Antibody | |
| Antibody: | 7D10 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | BAK L100A |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >7LK4_F|Chain F[auth B], H[auth D], J[auth F], L[auth H]|7D10 antibody VH fragment|Rattus norvegicus (10116) GSEVELVESGGDLVQPGRSLKLSCAASGFTFSNLAMAWVRQTPTKGLEWVASISPAGITTYYRDSVKGRFTISRDNARNTQYLQMDSLRSEDTATYYCARHTGKSSFFDYWGQGVMVTVSSGSENLYFQ |
Light Chain: E
Mutation: NULL
| >7LK4_E|Chain E[auth A], G[auth C], I[auth E], K[auth G]|7D10 antibody VL fragment|Rattus norvegicus (10116) GSDVQMTQSPSYLAASPGESVSISCKATENINTYLAWYQAKPGKTTKLLLYSGSTLQSGTPSRFSGSGSGTDFTLTISSLEPEDFAVYYCQQHNEYPLTFGSGTKLEIK |
Antigen
Chain: P
Mutation: L78A/C144S
| >7LK4_P|Chain A[auth P], B[auth Q], C[auth R], D[auth S]|Bcl-2 homologous antagonist/killer|Homo sapiens (9606) SEEQVAQDTEEVFRSYVFYRHQQEQEAEGVAAPADPEMVTLPLQPSSTMGQVGRQLAIIGDDINRRYDSEFQTMLQHAQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGFLGQVTRFVVDFMLHHSIARWIAQRGGWVAALNLGNG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition E: TYR32 TYR49 SER50 THR53 HIS91 ASN92 GLU93 TYR94 F: ALA33 SER50 ILE51 SER52 PRO53 ALA54 ILE56 THR57 TYR59 HIS99 GLY101 LYS102 SER103 SER104 PHE105 P: GLN47 GLU48 ALA49 GLU50 GLY51 VAL52 ALA53 ALA54 PRO55 ALA56 ASP57 PRO58 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
