Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4127 |
| PDBID: | 7LF7 |
| Chains: | AB_M |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.03 |
| Reference: | 10.1038/s42003-021-02387-5 |
| Antibody | |
| Antibody: | 6D12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Apolipoprotein L1 NTD |
| Antigen mutation: | No |
| Durg Target: | O14791 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7LF7_A|Chain A|Fab 6D12 heavy chain|Homo sapiens (9606) EVMLVESGGGLVRPGGSLKLSCTASGFTFSRCAMSWVRQTPEKRLEWVSAISRDSTYTYYSDSVKGRFTVSRDNAKNTLYLQMSSLRSEDTAMYYCARQIDDYYVDALDYWGQGTSVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: B
Mutation: NULL
| >7LF7_B|Chain B, E[auth L]|Fab 6D12 light chain|Homo sapiens (9606) DVQITQSPSYLAASPGETITINCRASKIISKYLAWYQEKPGKTIKLLIYSGFTLQSGIPSRFSGSGSGTDFTLTISSLEPEDFAMYYCQQHNEYPLTFGAGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: M
Mutation: NULL
| >7LF7_M|Chain D[auth K], F[auth M]|Apolipoprotein L1|Homo sapiens (9606) MDYKDDDDKGENLYFQGSDPESSIFIEDAIKYFKEKVSTQNLLLLLTDNEAWNGFVAAAELPRNEADELRKALDNLARQMIMKDKNWHDKGQQYRNWFLKEFPRLKSELEDNIRRLRALADGVQKVHKGT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: ARG53 TYR57 TYR59 ASP102 TYR103 TYR104 ASP106 B: LYS27 ILE28 ILE29 SER30 LYS31 TYR32 SER50 HIS91 ASN92 GLU93 M: GLU92 TRP94 ASN95 GLY96 VAL98 ALA99 ALA100 GLU102 LEU103 PRO104 ARG105 ASN106 GLU107 ALA108 ASP109 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
