Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4121 |
| PDBID: | 7LF2 |
| Chains: | FH_B |
| Organism: | Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.72 |
| Reference: | 10.1038/s42003-021-02444-z |
| Antibody | |
| Antibody: | mAb4 Full-length |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Arginase-1 (hArg1) |
| Antigen mutation: | No |
| Durg Target: | P05089 |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >7LF2_F|Chain B[auth D], D[auth F], E[auth G], I[auth K], J[auth O], K[auth P]|mAb4 monoclonal antibody heavy chain|Homo sapiens (9606) EVQLVQSGAEVKKPGASVKVSCKASGYTFTDYYMNWVRQAPGQGLEWIGVISPYNGGTTYNQKFKGKATLTVDKSTSTAYMELSSLRSEDTAVYYCVYDLYYFDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPPCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGK |
Light Chain: H
Mutation: NULL
| >7LF2_H|Chain C[auth E], N[auth H], O[auth I], P[auth L], Q[auth M], R[auth N]|mAb4 monoclonal antibody light chain|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCRASQRISNDLHWYQQKPGQAPRLLIKYASQSISGIPSRFSGSGSGTDFTLTISSLEPEDFAVYYCQQSNSWPRTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7LF2_B|Chain A, F[auth B], G[auth C], H[auth J], L[auth Q], M[auth R]|Arginase-1|Homo sapiens (9606) MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPIHLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIMEVNPSLGKTPEEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition F: THR30 ASP31 TYR32 TYR33 VAL50 SER52 TYR54 ASN55 GLY57 THR58 THR59 ASP99 LEU100 TYR101 TYR102 H: SER30 ASN31 ASP32 LYS49 TYR50 GLN53 SER67 ASN92 TRP94 ARG96 B: GLY22 GLY23 GLU26 THR29 VAL30 ARG32 LYS33 ALA34 GLY35 LEU37 GLU38 LYS39 LYS41 GLU42 PRO280 SER281 GLY283 LYS284 THR285 PRO286 GLU287 VAL289 THR290 VAL293 ASN294 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
