Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4102 |
| PDBID: | 7LEY |
| Chains: | HI_C |
| Organism: | Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.05 |
| Reference: | 10.1038/s42003-021-02444-z |
| Antibody | |
| Antibody: | mAb5 Full-length |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Arginase-1 (hArg1) |
| Antigen mutation: | No |
| Durg Target: | P05089 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7LEY_H|Chain B[auth D], H[auth G], I[auth H]|mAb5 heavy chain|Homo sapiens (9606) EVQLVESGGGVVRPGGSLRLSCAASGFTFDDYGMTWVRQAPGKGLEWVSGINWNGGSTGYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTALYHCARDRRRGSYGSDAFDIWGQGTMVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLESDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMNTNGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK |
Light Chain: I
Mutation: NULL
| >7LEY_I|Chain C[auth E], D[auth F], E[auth I]|mAb5 light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKVPQLLISAASTLQSGVPSRFSGSGSGTDFTLTISSLQPEDVATYYCQKYNSAPRTFGQGTKVEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >7LEY_C|Chain A, F[auth B], G[auth C]|Arginase-1|Homo sapiens (9606) MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPIHLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIMEVNPSLGKTPEEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition I: SER30 ASN31 TYR32 SER49 ALA50 THR53 GLN55 SER93 H: ASP30 ASP31 ASN52 TRP53 ASN54 GLY55 GLY56 SER57 ARG100 ARG101 ARG102 GLY103 SER104 TYR105 GLY106 SER107 ASP108 C: SER16 LYS17 GLN19 PRO20 ARG21 GLY22 GLY23 GLU25 GLU26 PRO54 PHE55 ALA56 ASP57 ILE58 PRO59 ASN60 LYS68 ASN69 PRO280 SER281 LEU282 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
