Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4095 |
| PDBID: | 7LEX |
| Chains: | DE_B |
| Organism: | Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.60 |
| Reference: | 10.1038/s42003-021-02444-z |
| Antibody | |
| Antibody: | mAb1 Full-length |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Arginase-1 (hArg1) |
| Antigen mutation: | No |
| Durg Target: | P05089 |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >7LEX_D|Chain B[auth D], N[auth F], O[auth G], P[auth K], Q, R|mAb1 heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYGISWVRQAPGQGLEWMGWISAYNGNTNYAQKLQGRVTMTTDTSTSTAYMELRSLRSDDTAVYYCAREGAYGYRSPYHNWFDPWGQGTLVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSRTPGK |
Light Chain: E
Mutation: NULL
| >7LEX_E|Chain C[auth E], F[auth H], G[auth I], I[auth L], L[auth O], M[auth P]|mAb1 light chain|Homo sapiens (9606) EIVMTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYYCQQHSLLPRTFGGGTKVEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >7LEX_B|Chain A, D[auth B], E[auth C], H[auth J], J[auth M], K[auth N]|Arginase-1|Homo sapiens (9606) MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPIHLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIMEVNPSLGKTPEEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition D: TRP50 TYR54 ASN55 ASN57 THR58 ASN59 TYR102 GLY103 TYR104 ARG105 SER106 PRO107 TYR108 ASN110 TRP111 E: GLN27 SER28 SER30 SER31 TYR32 TYR49 ASP50 ASN53 SER67 GLY68 SER92 LEU93 LEU94 ARG96 B: SER16 LYS17 GLN19 PRO20 ARG21 GLY22 GLY23 GLU25 GLU26 ASP57 PRO59 PRO63 LYS68 ASN69 HIS126 ASP128 ASN130 THR136 SER137 ASN139 HIS141 GLY142 ASP181 VAL182 ASP183 PRO184 GLU186 GLY245 THR246 PRO247 VAL248 VAL249 SER281 LEU282 LYS284 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
