Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 409 |
| PDBID: | 2ZNX |
| Chains: | A_Y |
| Organism: | Mus musculus, synthetic construct, Gallus gallus |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1021/bi300455t |
| Antibody | |
| Antibody: | 10 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Lysozyme C |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >2ZNX_A|Chain A, C[auth B]|ScFv|Mus musculus (10090) DIVLTQSPATLSVTPGNSVSLSCRASQSIGNNLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPYTFGGGTKLEITGGGGSGGGGSGGGGSDIQLQESGPSLVKPSQTLSLTCSVTGDSITSDYWSWIRKFPGNRLEYMGYVSYSGSTYYNPSLKSRISITRDTSKNQYYLDLNSVTTEDTATYYCANWDGDYWGQGTLVTVSAAHHHHHH |
Antigen
Chain: Y
Mutation: NULL
| >2ZNX_Y|Chain B[auth Y], D[auth Z]|Lysozyme C|Gallus gallus (9031) KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER28 GLY30 ASN31 ASN32 LYS49 TYR50 GLN53 SER91 ASN92 SER93 TYR96 ASP149 SER150 THR152 SER153 ASP154 TYR155 TYR172 SER174 TYR175 SER176 SER178 TYR180 ASP221 Y: LYS13 ARG14 HIS15 GLY16 ASP18 ASN19 TYR20 ARG21 TRP63 ARG73 ASN74 LEU75 CYS76 ASN77 THR89 ASN93 LYS96 LYS97 ILE98 SER100 ASP101 GLY102 ASN103 ALA107 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
