Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 403 |
| PDBID: | 2ZCH |
| Chains: | HL_P |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.83 |
| Reference: | 10.1016/j.jmb.2007.11.052 |
| Antibody | |
| Antibody: | 8G8F5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Prostate-specific antigen |
| Antigen mutation: | No |
| Durg Target: | P07288 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2ZCH_H|Chain C[auth H]|monoclonal antibody 8G8F5 Fab|Mus musculus (10090) QVQLQQSGDDLVKPGASVKLSCKASGYTFTTYYINWMRQRPGQGLEWIGRIAPASGTTYSSEMFKDKATLTVDTSSNTAYIQLSSLSSEDSAVYFCARADYGFNSGEAMDYWGQGTSVTVSSAKTTAPPVYPLAPVCGDTTGSSVTLGCLVKGYFPESVTLLWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIVPRDCGCKPC |
Light Chain: L
Mutation: NULL
| >2ZCH_L|Chain B[auth L]|monoclonal antibody 8G8F5 Fab|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCKASQSVDFDGDSYMNWYQQKPGQPPKLLIFAASNLASGIPARLSGSGSGTDFTLNIQPVEEEDAATYYCQQSNEDPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR |
Antigen
Chain: P
Mutation: NULL
| >2ZCH_P|Chain A[auth P]|Prostate-specific antigen|Homo sapiens (9606) IVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: PHE27 ASP28 TYR32 SER91 ASN92 TYR96 H: TYR27 THR28 THR30 THR31 TYR32 TYR33 ALA53 SER54 THR56 TYR58 ASP96 TYR97 GLY98 PHE99 P: HIS91 PRO92 LEU93 TYR94 ASP98 SER100 HIS101 THR125 GLN128 GLU129 LYS175 THR177 LYS178 PHE179 VAL232 HIS233 TYR234 ARG235 LYS236 TRP237 LYS239 ASP240 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
