Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4017 |
| PDBID: | 7KZX |
| Chains: | DC_B |
| Organism: | Shewanella oneidensis, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.00 |
| Reference: | 10.1085/jgp.202112873 |
| Antibody | |
| Antibody: | 2R Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cadmium and zinc efflux pump FieF |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >7KZX_D|Chain D, F|Fab2R heavy chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFTIYSSSIHWVRQAPGKGLEWVASIYSSSGSTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARQSYSGLSPRRHWSYGAMDYWGQGTLVTVFNQIKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: C
Mutation: NULL
| >7KZX_C|Chain C, E|Fab2R light chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQIWSWPLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7KZX_B|Chain A, B|Cadmium and zinc efflux pump FieF|Shewanella oneidensis (70863) MTQTSQYDFWVKLASRASVATALTLITIKLLAWLYSGSASMLASLTDSFADTLASIINFIAIRYAIVPADHDHRYGHGKAEPLAALAQSAFIMGSAFLLLFYGGERLLNPSPVENATLGVVVSVVAIVLTLALVLLQKRALAATNSTVVEADSLHYKSDLFLNAAVLLALVLSQYGWWWADGLFAVLIACYIGQQAFDLGYRSIQALLDRELDEDTRQRIKLIAKEDPRVLGLHDLRTRQAGKTVFIQFHLELDGNLSLNEAHSITDTTGLRVKAAFEDAEVIIHQDPVQVEPTTQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition C: SER1 GLN28 SER29 SER31 SER32 SER51 ARG67 ILE92 TRP93 SER94 TRP95 PRO96 D: TYR33 SER34 SER35 SER36 TYR55 SER57 SER58 SER60 TYR62 GLN102 SER103 TYR104 SER105 GLY106 ARG110 HIS112 TRP113 SER114 TYR115 GLY116 B: ASP215 ARG219 LEU222 ILE223 LYS225 GLU226 ASP227 PRO228 ARG229 VAL230 LEU231 GLY232 GLU252 LEU253 ASP254 ASN256 LEU257 GLU261 ILE265 THR268 ARG272 ALA276 VAL289 GLN290 VAL291 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
