Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4012 |
| PDBID: | 7KYL |
| Chains: | HL_E |
| Organism: | Mus musculus, Deer tick virus |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1084/jem.20210174 |
| Antibody | |
| Antibody: | POWV-80 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Powassan virus Envelope protein DIII |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7KYL_H|Chain A[auth H], D[auth X]|POWV-80 Fab heavy chain|Mus musculus (10090) EVKLVESEGGLVQPGSSMKVSCTASGFTFSHYYMAWVRQVPEKGLEWVANINYDGTSTYYLDSLKSRFIISRDNANNILYLQMSSLKSEDTAIYYCAREEGYGNPYPYWYFDVWGTGTTVTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIEPRVPIT |
Light Chain: L
Mutation: NULL
| >7KYL_L|Chain B[auth L], E[auth Y]|POWV-80 Fab light chain|Mus musculus (10090) QIVLTQSPAILSASPGEKVTMTCRASSSVSYMHWYQQKPGSSPKSWIYATSNLASGVPTRFSGSGSGTSYSLTISRVEAEDAATYYCQQRSSNPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >7KYL_E|Chain C[auth E], F[auth Z]|Envelope protein domain III|Powassan virus (58535) MKLKGTTYSMCDKTKFKWKRVPVDSGHDTVVMEVSYTGSDKPCRIPVRAVAHGVPTINVAMLITPNPTIETSGGGFIEMQLPPGDNIIYVGDLSQQWFQKGST |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: VAL4 PHE29 THR30 SER32 HIS33 TYR34 TYR35 TYR55 ASP56 ARG100 GLU102 GLY103 TYR104 GLY105 ASN106 PRO107 TYR108 TRP111 TYR112 ASP114 L: SER55 GLY56 E: ASP308 LYS309 THR310 ARG344 VAL346 HIS348 PRO351 THR352 ASN354 ILE383 TYR385 GLY387 ASP388 SER390 GLN391 GLN392 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
