Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 40 |
| PDBID: | 1FSK |
| Chains: | CB_A |
| Organism: | Betula pendula, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.4049/jimmunol.165.1.331 |
| Antibody | |
| Antibody: | BV16 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MAJOR POLLEN ALLERGEN BET V 1-A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >1FSK_C|Chain C, F, I, L|ANTIBODY HEAVY CHAIN FAB|Mus musculus (10090) QVQLQQPGTELVRPGASVILSCKASGYTFTSYWINWVKQRPGQGLEWVGNIFPSDSYTNYNQKFKDKATLTVDKSSSTAYMQVNSPTSEDSAVYYCTRGARDTWFAYWGQGTLVTVSVAKTTPPSVFPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: B
Mutation: NULL
| >1FSK_B|Chain B, E, H, K|IMMUNOGLOBULIN KAPPA LIGHT CHAIN|Mus musculus (10090) NIVLTQSPKSMSVSVGERVTLSCKASENVDTYVFWFQQKPDQSPKLLLYGPSNRYTGVPDRFTGSGSTTDFTLTISSVQAEDLADYHCGQSYSYPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >1FSK_A|Chain A, D, G, J|MAJOR POLLEN ALLERGEN BET V 1-A|Betula pendula (3505) GVFNYETETTSVIPAARLFKAFILDGDNLFPKVAPQAISSVENIEGNGGPGTIKKISFPEGLPFKYVKDRVDEVDHTNFKYNYSVIEGGPIGDTLEKISNEIKIVATPDGGSILKISNKYHTKGDHEVKAEQVKASKEMGETLLRAVESYLLAHSDAYN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: THR31 TYR32 PHE34 GLY50 SER91 TYR92 SER93 TYR94 TYR96 C: SER31 TYR32 TRP33 PHE52 SER54 ASP55 TYR57 ASN59 GLY99 ALA100 ARG101 ASP102 THR103 TRP104 A: LEU24 GLU42 ASN43 ILE44 GLU45 GLY46 ASN47 GLY48 GLY49 PRO50 GLY51 THR52 ILE53 LYS68 ARG70 ASP72 GLU73 HIS76 ILE86 GLU87 LYS97 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
