Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4 |
| PDBID: | 1AFV |
| Chains: | HL_A |
| Organism: | Human immunodeficiency virus 1, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.70 |
| Reference: | 10.1038/nsb0996-763 |
| Antibody | |
| Antibody: | 25.3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV TYPE 1 CAPSID PROTEIN(P24) |
| Antigen mutation: | No |
| Durg Target: | Q72497; P12497; P35963; O93182; P03369; P03367; P03366; P04587; P04585; P12499; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >1AFV_H|Chain D[auth H], F[auth K]|ANTIBODY FAB25.3 FRAGMENT (HEAVY CHAIN)|Mus musculus (10090) QVQLQQPGSVLVRPGASVKLSCKASGYTFTSSWIHWAKQRPGQGLEWIGEIHPNSGNTNYNEKFKGKATLTVDTSSSTAYVDLSSLTSEDSAVYYCARWRYGSPYYFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPK |
Light Chain: L
Mutation: NULL
| >1AFV_L|Chain C[auth L], E[auth M]|ANTIBODY FAB25.3 FRAGMENT (LIGHT CHAIN)|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCRASESVDNYGISFMNWFQQKPGQPPKLLIYAASNLGSGVPARFSGSGSGTDFSLNIHPMEEEDTAMYFCQQSKEVPLTFGAGTKVELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: NULL
| >1AFV_A|Chain A, B|HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 CAPSID PROTEIN|Human immunodeficiency virus 1 (11676) PIVQNLQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTSIL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASN31 TYR32 ILE34 PHE36 SER95 LYS96 GLU97 VAL98 LEU100 H: TRP33 HIS35 GLU50 ASN59 TRP99 TYR101 GLY102 SER103 PRO104 TYR105 A: THR72 ASN74 GLU75 GLU76 ALA77 ALA78 GLU79 ASP81 ARG82 LEU83 PRO85 VAL86 ARG100 SER102 LEU136 LYS140 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
