Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3986 |
| PDBID: | 7KPG |
| Chains: | HL_S |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.27 |
| Reference: | 10.1186/s13045-020-00989-w |
| Antibody | |
| Antibody: | 25 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tyrosine-protein phosphatase non-receptor type substrate 1(Signal regulatory protein α, SIRPα) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7KPG_H|Chain A[auth H]|Heavy chain of Fab 25 anti-SIRP-alpha antibody|Homo sapiens (9606) DVQLVESGGGVVRPGESLRLSCEASGFTFSSNAMSWVRQAPGKGLEWVAGISSGSDTYYGDSVKGRLTISRDNSKNILYLQMNSLTAEDTAVYYCARETWNHLFDYWGQGTLVTVSSASTKGPSVFPLAPGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: L
Mutation: NULL
| >7KPG_L|Chain B[auth L]|Light chain of Fab 25 anti-SIRP-alpha antibody|Homo sapiens (9606) ELTQPPSVSVSPGQTARITCSGGSYSSYYYAWYQQKPGQAPVTLIYSDDKRPSNIPERFSGSSSGTTVTLTITGVQAEDEADYYCGGYDQSSYTNPFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: S
Mutation: NULL
| >7KPG_S|Chain C[auth S]|Tyrosine-protein phosphatase non-receptor type substrate 1|Homo sapiens (9606) ELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGAITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: SER31 ASN32 ALA33 TRP47 GLY50 SER52 SER53 GLY54 ASP56 TYR58 GLU98 TRP100 HIS102 L: TYR27 SER28 SER29 TYR30 TYR31 SER49 ASP50 ASP51 LYS52 TYR90 GLN92 SER93 TYR95 ASN97 S: LEU30 ILE31 PRO32 VAL33 GLY34 PRO35 GLN52 LYS53 ASP65 LEU66 THR67 LYS68 ARG69 ASN70 MET72 LYS96 GLY97 SER98 PRO99 ASP101 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
