Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3962 |
| PDBID: | 7KLC |
| Chains: | HL_A |
| Organism: | Mus musculus, Homo sapiens, Human immunodeficiency virus 1 |
| Method: | XRD |
| Resolution (Å): | 4.30 |
| Reference: | 10.1128/mBio.00429-21 |
| Antibody | |
| Antibody: | M4H2K1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade A BG505 gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7KLC_H|Chain A[auth H]|M4H2K1 Fab heavy chain|Mus musculus (10090) EVKLEESGGGLVQPGGSMKLSCVASGITFSNSWMSWVRQSPEKGLEWVAEIRLKAQNYATHYAASVKGRFTISRDDSKSSVYLQMNNLRPEDTGIYYCTTPLGGYFDMDYWGQGTSLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDK |
Light Chain: L
Mutation: NULL
| >7KLC_L|Chain B[auth L]|M4H2K1 Fab light chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCRASESVDIYGISFMNWFQQRPGQPPKLLIYAASNRGSGVPARFSGSGSGTDFSLNIHPMEEDDTAMYFCQQSKEVPWTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7KLC_A|Chain E[auth A]|HIV-1 clade A BG505 gp120,HIV-1 clade A BG505 gp120|Human immunodeficiency virus 1 (11676) VWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVGAGNCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCNVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: THR28 ASN31 TRP33 ALA52 GLN53 ASN54 ALA56 HIS58 GLY98 TYR99 L: ILE27 TYR28 ILE30 PHE32 LYS92 GLU93 VAL94 TRP96 A: ILE277 THR278 ARG350 LYS351 HIS352 PHE353 GLY354 ASN355 ASN356 THR357 ILE359 ARG360 THR394 ILE396 SER397 ARG456 SER460 THR461 ASN462 SER463 THR464 THR465 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
