Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3948 |
| PDBID: | 7KI4 |
| Chains: | DC_E |
| Organism: | Henipavirus nipahense, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.90 |
| Reference: | 10.1038/s41594-021-00584-8 |
| Antibody | |
| Antibody: | 12B2 Full-length |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | henipaviruses (HNVs) fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >7KI4_D|Chain C[auth D], F[auth G], I[auth H]|12B2 heavy chain|Mus musculus (10090) QVQLKESGPGLVAPSQSLSITCTVSGFSLASYGVHWVRQPPGKGLEWLGVIWTGGSTNYNSALMSRLSINRDNSKSQVFLKLNSLQTDDTAIYYCARDRGYGYGGFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK |
Light Chain: C
Mutation: NULL
| >7KI4_C|Chain B[auth C], E[auth F], H[auth L]|12B2 Fab light chain|Mus musculus (10090) DIQMTQSPASLSASVGETVTITCRASENIYSYLAWYQHKQGKSPQLLVYNAKSLAEGVPSRFSGSGSGTQFSLKINSLQPEDFGSYYCQHHYGTPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >7KI4_E|Chain A, D[auth B], G[auth E]|Fusion glycoprotein F0|Nipah virus (3052225) MVVILDKRCYCNLLILILMISECSVGILHYEKLSKIGLVKGVTRKYKIKSNPLTKDIVIKMIPNVSNMSQCTGSVMENYKTRLNGILTPIKGALEIYKNNTHDLVGDVRLAGVIMAGVAIGIATAAQITAGVALYEAMKNADNINKLKSSIESTNEAVVKLQETAEKTVYVLTALQDYINTNLVPTIDKISCKQTELSLDLALSKYLSDLLFVFGPNLQDPVSNSMTIQAISQAFGGNYETLLRTLGYATEDFDDLLESDSITGQIIYVDLSSYYIIVRVYFPILTEIQQAYIQELLPVSFNNDDSEWISIVPNFILVRNTLISNIEIGFCLITKRSVICNQDYATPMTNNMRECLTGSTEKCPRELVVSSHVPRFALSNGVLFANCISVTCQCQTTGRAISQSGEQTLLMIDNTTCPTAVLGNVIISLGKYLGSVNYNSEGIAIGPPVFTDKVDISSQISSMNQSLQQSKDYIKEAQRLLDTVNPSMKQIEDKIEEILSKIYHIENEIARIKKLIGEAPGGIEGRKLKETAAAKFERQHMDS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition C: ILE2 GLU27 TYR30 SER31 TYR32 ASN50 TYR92 GLY93 THR94 D: TRP52 GLY54 SER56 ASN58 TYR101 GLY102 TYR103 E: ASN67 MET68 GLN70 CYS71 ILE187 ASP188 LYS189 ILE190 SER191 CYS192 LYS193 GLN194 LEU197 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
