Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3933 |
| PDBID: | 7KFH |
| Chains: | HK_B |
| Organism: | Ebola virus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.80 |
| Reference: | 10.1016/j.celrep.2021.108984 |
| Antibody | |
| Antibody: | EBOV-437 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus Virion spike glycoprotein 1 |
| Antigen mutation: | No |
| Durg Target: | Q05320; P00750; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7KFH_H|Chain D[auth G], H, L[auth I]|Antibody Fab EBOV-437 heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGGTFSRDIFSWVRQAPGQGLEWMGGIIPIFGTANYAQKFQGRVTITADKSTSTVFMELSGLRSEDTAVYFCARGPPLRGERSWFGESEKYDYFYMDVWGKGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: K
Mutation: NULL
| >7KFH_K|Chain B[auth J], F[auth K], J[auth L]|Antibody Fab EBOV-437 light chain|Homo sapiens (9606) QIVLTQSPGTLSLSPGERATLSCRASQSVSNSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLSISRLEPEDFAVYYCQQYGTSPCTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLRSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7KFH_B|Chain A, E[auth B], I[auth C]|Virion spike glycoprotein 1|Ebola virus (1570291) MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSVTKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYASGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| K: SER95 H: SER30 ARG31 ILE52 PRO53 ILE54 PHE55 GLY56 THR57 THR71 ALA72 ASP73 LYS74 SER75 GLY104 GLU105 ARG106 SER107 TRP108 PHE109 GLY110 GLU111 SER112 GLU113 LYS114 TYR115 ASP116 B: PRO116 ASP117 THR144 PRO146 GLN221 ALA222 THR223 GLY224 THR227 GLU229 THR230 GLU231 LEU233 TYR241 VAL242 GLN243 LEU256 THR259 ILE260 LYS265 THR269 THR270 GLY271 LYS272 LEU273 ILE274 TRP275 LYS276 VAL277 ASN278 ILE281 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
