Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3931 |
| PDBID: | 7KFE |
| Chains: | GI_B |
| Organism: | Homo sapiens, Bundibugyo virus |
| Method: | EM |
| Resolution (Å): | 6.60 |
| Reference: | 10.1016/j.celrep.2021.108984 |
| Antibody | |
| Antibody: | BDBV-329 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Bundibugyo virus spike glycoprotein 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >7KFE_G|Chain A[auth G], C[auth H]|Antibody BDBV-329 heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGGTFDTYAISWVRQAPGQGLEWMGGIIPVLGIVDYAQKFQGRVTITAAKFTNIAYMELSSLRSEDAAVYYCARGLRSLSPRGQEGPTPAPGWRRAQYHYYYMDVWGTGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCD |
Light Chain: I
Mutation: NULL
| >7KFE_I|Chain B[auth I], D[auth J]|Antibody BDBV-329 light chain|Homo sapiens (9606) EIVMTQSPGTLSLSPGERATLSCRASQSVSSNYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGPDFTLTISRLEPEDFAVYYCQQYGSSPGTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLRSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7KFE_B|Chain E[auth A], G[auth B], I[auth C]|Spike glycoprotein 1|Bundibugyo ebolavirus (565995) MVTSGILQLPRERFRKTSFFVWVIILFHKVFPIPLGVVHNNTLQVSDIDKLVCRDKLSSTSQLKSVGLNLEGNGVATDVPTATKRWGFRAGVPPKVVNYEAGEWAENCYNLDIKKADGSECLPEAPEGVRGFPRCRYVHKVSGTGPCPEGYAFHKEGAFFLYDRLASTIIYRSTTFSEGVVAFLILPETKKDFFQSPPLHEPANMTTDPSSYYHTVTLNYVADNFGTNMTNFLFQVDHLTYVQLEPRFTPQFLVQLNETIYTNGRRSNTTGTLIWKVNPTVDTGVGEWAFWENKKNFTKTLSSEELSVIFVPIDISESTEPGPLTNTTRGAANLLTGSRRTRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition G: THR28 ASP30 THR31 VAL54 LEU55 GLY56 ILE57 VAL58 TYR60 PHE75 SER102 SER104 PRO105 ARG106 GLN108 GLU109 PRO113 ALA114 PRO115 GLY116 ARG118 ARG119 ALA120 GLN121 B: LYS114 ALA116 ASP117 GLY118 GLY143 THR144 VAL221 ASP223 ASN224 MET229 ASN231 LEU233 GLN235 HIS238 LEU239 TYR241 GLN243 LEU256 THR259 ILE260 ASN263 LEU273 ILE274 TRP275 LYS276 VAL277 ASN278 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
