Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3924 |
| PDBID: | 7KF9 |
| Chains: | GJ_A |
| Organism: | Homo sapiens, Ebola virus |
| Method: | EM |
| Resolution (Å): | 4.40 |
| Reference: | 10.1016/j.celrep.2021.108984 |
| Antibody | |
| Antibody: | EBOV-296 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus Virion spike glycoprotein 1 |
| Antigen mutation: | No |
| Durg Target: | Q05320; P00750; |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >7KF9_G|Chain A[auth G], E[auth H], I|Antibody Fab EBOV-296 heavy chain|Homo sapiens (9606) MELGLRWVFLVAILEGVQCQVQLVESGPGLVKPSQTLSLICTVSGGSISSGDFYWSWIRQPPGKGLEWIGFFYYSGITYYNPSLKSRVSISRDTSTNQFSLKLRSVTAADTAVYYCARVRGGRITLGQGDMYYYSGMDVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCD |
Light Chain: J
Mutation: NULL
| >7KF9_J|Chain D[auth J], H[auth K], L|Antibody Fab EBOV-296 light chain|Homo sapiens (9606) MGWSCIILFLVATATGVHQSVLTQPPSASGTPGQRVSISCSGSRSNIGGNTVDWYQQLPGTDPKLLIYSNDQRPSGVPDRFSGSKSGTSASLAISGLQSEDEADYYCAAWDDSLNGHWVFGGGTKLTVLQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >7KF9_A|Chain B[auth A], F[auth B], J[auth C]|Virion spike glycoprotein 1|Ebola virus (1570291) MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSVTKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYASGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: ASP33 TYR54 ARG101 GLY102 GLY103 ARG104 ILE105 THR106 GLY108 GLN109 GLY110 ASP111 MET112 TYR113 TYR114 TYR115 J: ARG26 GLY31 ASN32 THR33 TYR50 SER51 ASN52 ASP53 GLN54 ARG55 PRO56 SER57 LYS67 SER68 GLY69 ASP94 A: THR223 GLU229 GLU231 GLN243 ARG247 PHE252 LEU256 THR259 ILE260 SER263 LYS265 ARG266 SER267 ASN268 THR269 THR270 GLY271 LYS272 LEU273 ILE274 TRP275 LYS276 VAL277 ASN278 GLU280 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
