Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3922 |
| PDBID: | 7KF1 |
| Chains: | AB_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.45 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | bH1(CDR H3 loop design 14_0130) Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Isoform L-VEGF206 of Vascular endothelial growth factor A (VEGF-A) |
| Antigen mutation: | No |
| Durg Target: | P15692 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7KF1_A|Chain A, D, G, J[auth H]|anti-VEGF-A Fab bH1 heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCAKLGIGYYYYGMDVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTGHHHHHHHHG |
Light Chain: B
Mutation: NULL
| >7KF1_B|Chain B, E, H[auth I], K[auth L]|anti-VEGF-A Fab bH1 light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDIPRSISGYVAWYQQKPGKAPKLLIYWGSYLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >7KF1_C|Chain C, F, I[auth J], L[auth V]|Isoform L-VEGF206 of Vascular endothelial growth factor A|Homo sapiens (9606) APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TYR33 ARG50 TYR56 ARG58 GLY98 TYR99 B: ARG27 SER28 ILE29 SER30 GLY31 TYR32 TRP50 TYR53 HIS91 TYR92 THR93 THR94 C: LYS48 PRO49 GLN79 MET81 ARG82 ILE83 HIS86 GLN87 GLY88 GLN89 HIS90 ILE91 GLY92 GLU93 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
