Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3915 |
| PDBID: | 7KEX |
| Chains: | HK_B |
| Organism: | Homo sapiens, Ebola virus |
| Method: | EM |
| Resolution (Å): | 4.25 |
| Reference: | 10.1016/j.celrep.2021.108984 |
| Antibody | |
| Antibody: | EBOV-293 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola virus Virion spike glycoprotein 1 |
| Antigen mutation: | No |
| Durg Target: | Q05320; P00750; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7KEX_H|Chain B[auth G], F[auth H], J[auth I]|Antibody Fab EBOV-293 heavy chain|Homo sapiens (9606) MELGLRWVFLVAILEGVQCQVQLVQSGAEVKKPGSSVKVSCKASGGTFIDYTYNWVRQAPGQGLVWMGRVVPGLGLSSYAQQFEDRVTITAAKSTSTVYMELRGLTSEDTAMYYCARDTASFGWLPFAFWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCD |
Light Chain: K
Mutation: NULL
| >7KEX_K|Chain A[auth J], E[auth K], I[auth L]|Antibody Fab EBOV-293 light chain|Homo sapiens (9606) MGWSCIILFLVATATGVHQTVMTQSPGTLSLSPGERATLSCGVSQSISSTYFAWYQQKVGQAPRLLIYGASNRASGIPDRFSGSASGTDFTLTISRVEPEDFAVYYCQQYGSSPTFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7KEX_B|Chain C[auth A], G[auth B], K[auth C]|Virion spike glycoprotein 1|Ebola virus (1570291) MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSVTKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYASGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition K: GLN1 THR2 GLN27 SER28 ILE29 TYR33 GLN91 GLY93 SER94 SER95 H: THR33 TRP47 ARG50 VAL51 VAL52 GLY54 LEU55 GLY56 LEU57 SER58 SER59 TYR60 GLU65 ASP66 THR69 ILE70 THR71 ALA72 ALA73 ALA101 GLY104 LEU106 B: VAL242 LEU256 THR259 ILE260 SER263 GLY264 LYS265 ARG266 SER267 ASN268 THR269 THR270 GLY271 LYS272 LEU273 ILE274 TRP275 LYS276 VAL277 ASN278 PRO279 GLU280 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
