Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 39 |
| PDBID: | 1FNS |
| Chains: | HL_A |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1038/79639 |
| Antibody | |
| Antibody: | NMC4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | von Willebrand factor (VWF) A1 DOMAIN |
| Antigen mutation: | Yes |
| Durg Target: | P04275 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >1FNS_H|Chain B[auth H]|IMMUNOGLOBULIN NMC-4 IGG1|Mus musculus (10090) QVQLKESGPGLVAPSQSLSITCTVSGFSLTDYGVDWVRQPPGKGLEWLGMIWGDGSTDYNSALKSRLSITKDNSKSQVFLKMNSLQTDDTARYYCVRDPADYGNYDYALDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCG |
Light Chain: L
Mutation: NULL
| >1FNS_L|Chain A[auth L]|IMMUNOGLOBULIN NMC-4 IGG1|Mus musculus (10090) DIQMTQSPSSLSASLGDRVTISCSASQDINKYLNWYQQKPDGAVKLLIFYTSSLHSGVPSRFSGSGSGTDYSLTISNLEPEDIATYYCQQYEKLPWTFGGGTKLEVKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: I546V
| >1FNS_A|Chain C[auth A]|VON WILLEBRAND FACTOR|Homo sapiens (9606) MYCSRLLDLVFLLDGSSRLSEAEFEVLKAFVVDMMERLRVSQKWVRVAVVEYHDGSHAYIGLKDRKRPSELRRIASQVKYAGSQVASTSEVLKYTLFQIFSKIDRPEASRIALLLMASQEPQRMSRNFVRYVQGLKKKKVIVIPVGIGPHANLKQIRLIEKQAPENKAFVLSSVDELEQQRDEIVSYLCDLAPEAP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASN30 TYR32 GLU92 LEU94 TRP96 H: THR244 MET264 TRP266 GLY267 ASP268 SER270 THR271 ASP272 ASP315 TYR316 GLY317 ASN318 TYR319 TYR321 A: PRO627 GLN628 ARG629 SER631 ARG632 ASN633 PHE634 VAL635 ARG636 ASN658 LYS660 GLN661 LEU664 LYS667 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
