Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3851 |
| PDBID: | 7K78 |
| Chains: | L_H |
| Organism: | Saccharomyces cerevisiae, Saccharomyces cerevisiae S288C, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1038/s41467-021-21985-9 |
| Antibody | |
| Antibody: | 7K78 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Histone H2B.1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: L
Mutation: NULL
| >7K78_L|Chain K, L|scFv|Mus musculus (10090) MKSSHHHHHHENLYFQSNAMEVQLQQSGPELVEPGTSVKMPCKASGYTFTSYTIQWVKQTPRQGLEWIGYIYPYNAGTKYNEKFKGKATLTSDKSSSTVYMELSSLTSEDSAVYYCARKSSRLRSTLDYWGQGTSVTVSSGGGGSGGGGSGGGGSMDIKMTQSPSSMHASLGERVTITCKASQDIRSYLSWYQQKPWKSPKTLIYYATSLADGVPSRFSGSGSGQDFSLTINNLESDDTATYYCLQHGESPYTFGSGTKLEIKRA |
Antigen
Chain: H
Mutation: NULL
| >7K78_H|Chain D, H|Histone H2B.1|Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (559292) MSAKAEKKPASKAPAEKKPAAKKTSTSTDGKKRSKARKETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSSTQA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR54 ARG120 SER122 ARG124 ARG126 SER127 ASP130 ARG188 TYR207 SER211 ASP214 H: GLN51 THR52 HIS53 GLU109 LEU110 LYS112 HIS113 SER116 GLU117 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
