Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 3849 |
| PDBID: | 7K78 |
| Chains: | K_C |
| Organism: | Saccharomyces cerevisiae, Saccharomyces cerevisiae S288C, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1038/s41467-021-21985-9 |
| Antibody | |
| Antibody: | 7K78 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Histone H2A.1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: K
Mutation: NULL
| >7K78_K|Chain K, L|scFv|Mus musculus (10090) MKSSHHHHHHENLYFQSNAMEVQLQQSGPELVEPGTSVKMPCKASGYTFTSYTIQWVKQTPRQGLEWIGYIYPYNAGTKYNEKFKGKATLTSDKSSSTVYMELSSLTSEDSAVYYCARKSSRLRSTLDYWGQGTSVTVSSGGGGSGGGGSGGGGSMDIKMTQSPSSMHASLGERVTITCKASQDIRSYLSWYQQKPWKSPKTLIYYATSLADGVPSRFSGSGSGQDFSLTINNLESDDTATYYCLQHGESPYTFGSGTKLEIKRA |
Antigen
Chain: C
Mutation: NULL
| >7K78_C|Chain C, G|Histone H2A.1|Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (559292) MSGGKGGKAGSAAKASQSRSAKAGLTFPVGRVHRLLRRGNYAQRIGSGAPVYLTAVLEYLAAEILELAGNAARDNKKTRIIPRHLQLAIRNDDELNKLLGNVTIAQGGVLPNIHQNLLPKKSAKATKASQEL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| K: THR50 THR52 SER53 TYR54 TYR74 TYR76 ASN77 SER122 SER123 ARG124 LEU125 ARG126 ARG188 TYR190 GLU251 SER252 C: TYR59 GLU63 GLU66 LEU67 ASN70 ARG73 ARG90 ASN91 ASP92 ASP93 GLU94 LEU95 ASN96 LYS97 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
