Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 381 |
| PDBID: | 2X7L |
| Chains: | HL_N |
| Organism: | synthetic construct, Human immunodeficiency virus type 1 BH10 |
| Method: | XRD |
| Resolution (Å): | 3.17 |
| Reference: | 10.1073/pnas.0914946107 |
| Antibody | |
| Antibody: | 2X7L Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 protein REV |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2X7L_H|Chain A, C, E, G, H, J|FAB HEAVY CHAIN|synthetic construct (32630) QEQLVESGGRLVTPGTALTLTCKVSGFSLSGFWLNWVRQAPGKGLEWVGAIYRGSGSEWYASWAKGRFTISDTSTTVTLKLTSPTTEDTATYFCAADTTDNGYFTIWGPGTLVTVSSASTKGPSVFPLAPSAKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKAEPKSCDKTRGHHHHHH |
Light Chain: L
Mutation: NULL
| >2X7L_L|Chain B, D, F, I, K, L|FAB LIGHT CHAIN|synthetic construct (32630) ELVMTQTPSSVSEPVGGTVTIKCQASQSISSWLSWYQQKPGQPPKLLIYDASNLASGVPSRFMGSGSGTEYTLTISGVQREDAATYYCLGGYPAASYRTAFGGGTELEIIRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: N
Mutation: NULL
| >2X7L_N|Chain M, N, O, P, Q, R|PROTEIN REV|HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 BH10 (11678) AGRSGDSDEDLLKAVRLIKFLYQSNPPPNPEGTRQARRNRRRRWRERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILVESPTVLESGAKE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 TRP33 TYR52 GLY54 SER55 GLY56 SER57 GLU58 TRP59 TYR60 THR99 ASP100 ASN101 L: SER28 SER30 SER31 TRP32 ASP50 TYR92 ALA94 ALA95 SER96 TYR97 N: ASP11 LYS14 ALA15 LEU18 PHE21 LEU22 SER25 ASN26 ARG48 GLN51 ILE55 ARG58 ILE59 THR62 TYR63 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
